Fig 1: Let-7a-5p could bind to DUSP7 in BC cells. (A) The binding sites between let-7a-5p and DUSP7 were predicted by Starbase. (B, C) Luciferase activity was determined in MCF-7/PTX and MDA-MB-231/PTX cells co-transfected with miR-NC or let-7a-5p and DUSP7 3ʹ UTR-WT or DUSP7 3ʹ UTR-MUT. (D, E) The abundance of DUSP7 mRNA and protein was measured in PTX-sensitive or resistant BC tissues by qRT-PCR and Western blot assay. (F) Western blot assay was conducted to measure the abundance of DUSP7 in MCF-10A, MCF-7, MCF-7/PTX, MDA-MB-231 and MDA-MB-231/PTX cells. (G) Correlation analysis was performed to analyze the abundance of let-7a-5p and DUSP7 in PTX-resistant BC tissues. (H) The overexpression efficiency of let-7a-5p was assessed in MCF-7/PTX and MDA-MB-231/PTX cells transfected with miR-NC or let-7a-5p by qRT-PCR. MCF-7/PTX and MDA-MB-231/PTX cells were transfected with miR-NC, let-7a-5p, let-7a-5p + pcDNA or let-7a-5p + DUSP7. (I, J) Western blot assay was applied to detect the enrichment of DUSP7 in the above two cells. (K) The IC50 of PTX in the above two cells was measured by MTT assay. (L) Flow cytometry was applied to examine the apoptosis of the above MCF-7/PTX and MDA-MB-231/PTX cells. (M) Transwell invasion assay was conducted to detect the invasion of MCF-7/PTX and MDA-MB-231/PTX cells. (N, O) The abundance of autophagy-related proteins was detected in MCF-7/PTX and MDA-MB-231/PTX cells by Western blot assay. *P<0.05.
Fig 2: The enrichment of DUSP7 is modulated by circ-ABCB10/let-7a-5p axis in BC cells. (A, B) The protein expression of DUSP7 in MCF-7/PTX and MDA-MB-231/PTX cells transfected with si-NC, si-circ-ABCB10, si-circ-ABCB10 + anti-miR-NC or si-circ-ABCB10 + anti-let-7a-5p was determined by Western blot assay. *P<0.05.
Fig 3: Let-7a-5p could bind to DUSP7 in BC cells. (A) The binding sites between let-7a-5p and DUSP7 were predicted by Starbase. (B, C) Luciferase activity was determined in MCF-7/PTX and MDA-MB-231/PTX cells co-transfected with miR-NC or let-7a-5p and DUSP7 3ʹ UTR-WT or DUSP7 3ʹ UTR-MUT. (D, E) The abundance of DUSP7 mRNA and protein was measured in PTX-sensitive or resistant BC tissues by qRT-PCR and Western blot assay. (F) Western blot assay was conducted to measure the abundance of DUSP7 in MCF-10A, MCF-7, MCF-7/PTX, MDA-MB-231 and MDA-MB-231/PTX cells. (G) Correlation analysis was performed to analyze the abundance of let-7a-5p and DUSP7 in PTX-resistant BC tissues. (H) The overexpression efficiency of let-7a-5p was assessed in MCF-7/PTX and MDA-MB-231/PTX cells transfected with miR-NC or let-7a-5p by qRT-PCR. MCF-7/PTX and MDA-MB-231/PTX cells were transfected with miR-NC, let-7a-5p, let-7a-5p + pcDNA or let-7a-5p + DUSP7. (I, J) Western blot assay was applied to detect the enrichment of DUSP7 in the above two cells. (K) The IC50 of PTX in the above two cells was measured by MTT assay. (L) Flow cytometry was applied to examine the apoptosis of the above MCF-7/PTX and MDA-MB-231/PTX cells. (M) Transwell invasion assay was conducted to detect the invasion of MCF-7/PTX and MDA-MB-231/PTX cells. (N, O) The abundance of autophagy-related proteins was detected in MCF-7/PTX and MDA-MB-231/PTX cells by Western blot assay. *P<0.05.
Fig 4: The enrichment of DUSP7 is modulated by circ-ABCB10/let-7a-5p axis in BC cells. (A, B) The protein expression of DUSP7 in MCF-7/PTX and MDA-MB-231/PTX cells transfected with si-NC, si-circ-ABCB10, si-circ-ABCB10 + anti-miR-NC or si-circ-ABCB10 + anti-let-7a-5p was determined by Western blot assay. *P<0.05.
Supplier Page from Abcam for Anti-DUSP7 antibody