Western blot using antibody IQ377 (clone AC10) on 5 different MRSA strains. Staph. Aureus cells were boiled for 5 minutes, and then loaded onto 12% SDS-PAGE gels. Following SDS=-PAGE separation, proteins were blotted on to nitrocellulose membrane, before being incubated with antibody at 1/1,000 overnight. Bound antibody was detected with HRP-conjugated anti-mouse IgG. Clone AC10 binds to a protein of 76kD, consistent with the expected size of PBP2a.