Fig 1: Baicalin represses the translation of RSV M RNA by inducing the extra ribosomal release of the cognate repressor protein L13a. (A, B) Baicalin induced an increase in the release of L13a from ribosomes in mice RSV-infected lungs, as demonstrated by immunoblotting (A). This release was specific for L13a, as no effect was seen on another larger subunit protein L11 (B). Top and bottom refer to the released proteins and ribosome sediments, respectively, collected post-sucrose gradient centrifugation using the lung homogenates. Representative blots for one mouse in each group (n = 3) are shown. (C) Immunohistochemistry analysis of L13a, phosphorylated L13a, and RSV viral NS1 and M proteins in lung tissues of RSV-infected mice ± baicalin. Scale bar = 80 µm.