Fig 1: The translation of FMRP targets is limited by initiation rather than elongation rates. a) Ribosome footprints of FMRP targets Poe, osa, and HUWE1 in control and Fmr1 RNAi oocytes. b) Cumulative distribution plots showing nearly identical distributions of ribosome footprints for FMRP targets in wild-type and Fmr1 RNAi oocytes. c) Ribosome footprints of mouse FMRP targets Arid2, HUWE1, and Auts2. f) Cumulative distribution plot showing nearly identical distributions of ribosome footprints of Arid2, HUWE1, and Auts2 in wild-type vs FMRP-deficient tissue. e, f) Plots showing that all mouse FMRP targets as analyzed as in (d) have low K–S scores <0.3.
Fig 2: FMRP targets encoding large proteins are downregulated in the mouse cortex. a, a′) Profiles and quantification of capillary electrophoresis western experiments showing a partial reduction in the levels of FMRP targets Auts2 and Med13L and complete loss of FMRP in the Fmr1 KO mouse cortex as compared to wild-type controls. Insets show standard curves of western signal as a function of total protein concentration. b) Total protein levels of all biotin-labeled proteins in mouse cortex extracts. b′) A zoom-in of (b) showing large proteins >300 kDa. b″) Quantification of (b) showing a decrease in the levels of large proteins but not total proteins in Fmr1 KO mouse cortex samples. c) Quantification of capillary electrophoresis western experiments of indicated proteins performed on mouse cortex extracts from wild-type or Fmr1 KO animals. *P < 0.05, #P < 0.1.
Supplier Page from Abcam for Anti-AUTS2 antibody