Fig 1: Dysregulation of PC programming in Ncam-1 KO mice. (A) Volcano plot of relative transcript expression from Ncam-1 WT and KO PCs (n=3). All significantly (P<0.05) expressed genes are labeled red (enriched) or blue (downregulated) and all nonsignificantly different transcripts are gray. (B) Functional network analysis using the DAVID functional annotation tool of differentially expressed genes from PCs of Ncam-1 WT and KO mice. (C) Table of selected targets and quantitative RT-PCR of enriched in PCs of Ncam-1 WT mice compared with PCs of Ncam-1 KO mice with log2-fold change, fold change and P-value. (D) SCN4B in PCs and VMs of Ncam-1 WT and KO mice (n=3). Western blot analysis and quantification of adult brain (30 μg) and heart ventricular lysates (30 μg) probed for SCN4B. Vinculin was used as loading control. Data represent mean±s.e.m. *P<0.05, **P<0.01, ***P <0.001 (two-tailed Student's t-test) WT versus KO. NS, not significant.