Complement C3 Antibody NBP1-32080 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemComplement C3 Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNBP1-32080
TargetComplement C3
ImmunogenRecombinant protein encompassing a sequence within the C-terminus region of human Complement C3. The exact sequence is proprietary
NCBI Gene AliasesCPAMD1, HEL-S-62p, ASP, ARMD9, C3a, C3b, AHUS5
HostRabbit
FormatPBS, 1% BSA, 20% Glycerol
ReactivityHuman,Mouse,Rat
Quantity100 ul
ApplicationsIF/IHC,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
IsotypeIgG
NCBI Full Gene Namecomplement C3
ClonalityPolyclonal
PurityAntigen Affinity-purified
Molecular Weight187 kDa
Research AreaAngiogenesis, Apoptosis, Autoimmune Diseases, Immunology, Inflammation, Innate Immunity, Mesenchymal Stem Cell Markers, Neurodegeneration, Neuroscience, Signal Transduction
Antibody TypePrimary Antibodies
StorageAliquot and store at -20C or -80C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunofluorescence staining in rat kidney tissues?

Yes, the Complement C3 Antibody has been successfully used for immunofluorescence staining in rat kidney tissues, as demonstrated in a study where it was applied to detect complement C3 deposition in a rat model of membranous nephropathy ().

What species does this antibody react with, and how has it been utilized in research?

The Complement C3 Antibody is reactive with mouse, human, and rat species. It has been utilized in various applications, including Western blotting and immunohistochemistry, to study complement activation and its role in diseases such as glomerulonephritis ().

Is there any specific application where this antibody has shown effectiveness in scientific studies?

The antibody has shown effectiveness in immunohistochemistry, particularly in studies assessing renal pathology. For example, it was used to evaluate the deposition of complement components in kidney tissues, contributing to the understanding of immune injury mechanisms in renal diseases ().

What is the recommended storage condition for this antibody to maintain its stability?

The Complement C3 Antibody should be aliquoted and stored at -20°C or -80°C to maintain its stability, and it is important to avoid freeze-thaw cycles to preserve its functionality.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Combined Effects of Exercise and Vitamin D on Neuroinflammation, Blood-Brain Barrier Integrity, Oxidative Stress, and Cognitive Function in Nonpathological MiceInternational neurourology journalJuly 1, 2025Kim T, Kim D, Kim Y, Kim J, Kang S, Cho J.PA5-85660), anti-GLAST (Miltenyi Biotec, Germany; Cat. 130-118-483), anti-C3 (Novus Biologicals, USA; Cat. NBP1-32080), and anti-S100A10 (Thermo Fisher Scientific; Cat. PA5-95505). After phosphate-buffered saline washing,

(2) YAP induces a neonatal-like pro-renewal niche in the adult heartNature Cardiovascular ResearchMarch 21, 2024Rich Gang Li, Xiao Li, Yuka Morikawa, Francisco J. Grisanti-Canozo, Fansen Meng, Chang-Ru Tsai, Yi Zhao, Lin Liu, Jong Kim, Bing Xie, et al.antibody incubation. Primary antibodies used included C3 (1:200, Abcam, ab200999 and 1:200, Novus, NBP1-32080); C3ar1 (1:200, Novus, MAB10417-100); Tβ4 (1:200, Abcam, ab167650 and ab14335); CTnT-AF647 (1:200, BD Biosciences

(3) Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathwayRenal failureJanuary 1, 2023Liu H, Cheng H, Wang H, Wang Q, Yuan J.fluorescein isothiocyanate (FITC)-conjugated goat anti-rat IgG (ab6840, 1:1000; Abcam), rabbit anti-rat C3 (NBP1-32080, 1:200; Novus Biologicals, Shanghai, China) and mouse anti-rat C5b-9 (sc-66190, 1:500; Santa Cruz Biotechnology,

(4) Prenatal glucocorticoid exposure selectively impairs neuroligin 1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal FGFR1 axisCellular and molecular life sciences : CMLSMay 13, 2022Choi GE, Chae CW, Park MR, Yoon JH, Jung YH, Lee HJ, Han HJ.from Cell Signaling Technology, Inc. (Danvers, MA, USA). The antibodies of vGAT (#NBP2-20857) and C3 (#NBP1-32080) were purchased from Novus Biologicals (Littleton, CO, USA). The antibodies of DCX (doublecortin, #PA5-17428),

(5) Optogenetic stimulation of CA3 pyramidal neurons restores synaptic deficits to improve spatial short-term memory in APP/PS1 miceProg NeurobiolFebruary 1, 2022Yang Q, Song D, Xie Z, He G, Zhao J, Wang Z, Dong Z, Zhang H, Yang L, Jiang M, et al.

(6) C3d Elicits Neutrophil Degranulation and Decreases Endothelial Cell Migration, with Implications for Patients with Alpha-1 Antitrypsin DeficiencyBiomedicinesDecember 16, 2021Fee LT, Gogoi D, O'Brien ME, McHugh E, Casey M, Gough C, Murphy M, Hopkins AM, Carroll TP, McElvaney NG, et al.following primary antibodies were used for probing immuno-blots: rabbit anti-C3 (Novus Biologicals, NBP1-32080, 1 µg/mL); mouse monoclonal BPI (Santa Cruz Biotechnology, Dallas, TX, USA, H-10, 0.2 µg/mL); goat polyclonal

(7) Polyphenol Supplementation Reverses Age-Related Changes in Microglial Signaling CascadesInternational journal of molecular sciencesJune 14, 2021Jalloh A, Flowers A, Hudson C, Chaput D, Guergues J, Stevens SM Jr, Bickford PC.◦ C for 5 min, vortexed, and then placed on ice. CD11b (Novus, NB110-89474), Complement C3 (Novus, NBP1-32080), PGRMC1 (Novus, Centennial, CO, USA, NBP1-83220), Thioredoxin 1 (Cell Signaling, Danvers, MA, USA,

(8) Reduced expression of innate immunity-related genes in lymph node metastases of luminal breast cancer patientsScientific reportsMarch 3, 2021Popeda M, Markiewicz A, Stokowy T, Szade J, Niemira M, Kretowski A, Bednarz-Knoll N, Zaczek AJ.(Dako) for 5 min. The sections were incubated for 1 h at RT with polyclonal rabbit anti-C3 antibody (NBP1-32080, NOVUS Biologicals) diluted 1:250, envisioned by EnVision Kit, Rabbit/Mouse (Dako) and counterstained

(9) Specific contribution of mannose-binding lectin murine isoforms to brain ischemia/reperfusion injuryCellular & molecular immunologyMarch 1, 2020Neglia L, Oggioni M, Mercurio D, De Simoni MG, Fumagalli S.

(10) A non-retinoid antagonist of retinol-binding protein 4 rescues phenotype in a model of Stargardt disease without inhibiting the visual cycleThe Journal of biological chemistryJuly 20, 2018Racz B, Varadi A, Kong J, Allikmets R, Pearson PG, Johnson G, Cioffi CL, Petrukhin K.

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