Figure.2: Spectral Confocal Microscopy of CHO cells using HY-8H10 antibody. CHO cells were transiently transfected with an expression vector encoding CEACAM19. Binding of HY-8H10 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

Figure.1: FACS analysis of BOSC23 cells using HY-8H10 antibody. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM19 (Red curve) or an irrelevant protein (control transfectant). Binding of HY-8H10 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.

Figure.3: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or CEACAM19-21. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8:Tet2; green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified HY-8H10 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM19 transfected cells (red curve).