Figure 2: BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructswas tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1, 3, 4, 5 and 6: D14HD11; CEACAM7: BAC2; CEACAM8:Tet2; PSG: Brified GM-2H6 was tested on all CEACAM transfectants. A positive signal wasobtained only with CEACAM8 transfected cells (Red curve).