C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and staining buffer (autofluorescence) (left) or 0.06 µg of Syrian Hamster IgG Isotype Control, Alexa Fluor 532 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).