Western blot was performed using Anti-MYBPC3 Recombinant Rabbit Monoclonal Antibody (Product # 703574) and a 150 kDa band corresponding to MYBPC3 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated into cardiomyocytes (Lane 2) and tissue extracts of Mouse Heart (Lane 3), Mouse Skeletal Muscle (Lane 4), Rat Heart (Lane 5) and Rat Skeletal Muscle (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).