Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex 117618 from BioLegend

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Veterinary Research
July 29 2024

Fig 1: HbpA induces the secretion of IL-6, TNF-α, and MCP-1. A–C 3D4/21 macrophages were incubated with SC1401 or SC1401ΔhbpA at an MOI of 10. The bar graphs show the relative IL-6, TNF-α, and MCP-1 mRNA levels. D–F 3D4/21 macrophages were incubated with 2, 20, or 40 µg/mL rHbpA protein; 100 ng/mL LPS was used as the positive control, and 20 µg/mL pET-32a His-tag protein was used as the negative control. The bar graphs show the relative IL-6, TNF-α, and MCP-1 mRNA levels. The fold change in expression on the y-axis was calculated as the experimental group/negative control. G–I 3D4/21 macrophages were incubated with 0.2, 2, 20, or 40 µg/mL rHbpA protein; 100 ng/mL LPS was used as the positive control, and 20 µg/mL pET-32a His-tag protein was used as the negative control. The bar graphs show the secreted IL-6, TNF-α, and MCP-1 levels as determined by ELISA. All assays were performed three times.
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Adv Sci (Weinh)
July 06 2026

Fig 2: The thymus is the primary source of circulating IL‐7R‐enriched EVs. (a) qRT‐PCR analysis of IL‐7R expression in colon, thymus, bone marrow, brain, and liver tissues of mice. (b) Immunofluorescence evaluation of IL‐7R expression in the thymus. (c) Nanoflow cytometry analysis of IL‐7R levels in serum EVs. (d) TD‐EVs‐Normal and TD‐EVs‐Colitis were analyzed using negative‐staining TEM and western blotting detection of TSG101, CD9, and CD81. (e) Nanoflow cytometry analysis of IL‐7R levels in TD‐EVs‐Normal and TD‐EVs‐Colitis. (f) Western blotting detection of IL‐7R in TD‐EVs‐Normal and TD‐EVs‐Colitis. (g) ELISA quantification of LPS levels in serum and thymus. (h) LPS administration via tail vein injection in mice, LPS levels in serum and thymus were quantified using ELISA. (i) qRT‐PCR analysis of IL‐7R expression post‐LPS treatment. (j) Immunofluorescence of thymic IL‐7R post‐LPS treatment. (k) TD‐EVs‐LPS was analyzed using negative‐staining TEM and western blotting detection of TSG101, CD9, CD81, IL‐7R levels. (l) Ex vivo imaging of DiR‐labeled TD‐EVs‐Colitis and TD‐EVs‐LPS in colon, spleen, liver, femur, tibia, brain, thymus, and lung. (m) Neutrophils were incubated with PKH26‐labeled TD‐EVs‐Normal, TD‐EVs‐Colitis, TD‐EVs‐LPS, and EVs internalization was examined using laser scanning confocal microscopy. (n–q) NET formation assay: Neutrophils were treated with PBS, TD‐EVs‐Normal, TD‐EVs‐Colitis, TD‐EVs‐LPS, or PMA, with/without anti‐IL‐7R antibody. NETs were quantified. TD‐EVs‐Normal: EVs from thymus of normal mice; TD‐EVs‐Colitis: EVs from thymus of DSS‐induced colitis mice; TD‐EVs‐LPS: EVs from thymus of LPS‐treated mice; IL‐7R (‐): anti‐IL‐7R antibody. n = 4–5 mice per group; results are presented as the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: non‐significance.

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Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex

BioLegend's Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex is a Rat monoclonal antibody. The Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex Antibody was generated using TLR4 as the antigen. It reacts with Mouse. This antibody has been shown to work in applications such as: Precipitation, Block/Neutralize/Inhibit, Flow Cytometry, Immunohistochemistry, Immunohistochemistry - fixed, and Immunoprecipitation.

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Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex [MTS510];

Veterinary Research

Adv Sci (Weinh)

Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex from BioLegend

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BioLegend

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Ultra-LEAF™ Purified anti-mouse TLR4 (CD284)/MD2 Complex from BioLegend

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