Fig 2: Western blot analysis of Protein expression of A) GATA-3, B) β2-AR, GATA-3, STAT 4 & 6, and β-Actin, C) β2-AR intensity, D) GATA-3, STAT4 and STAT6 intensities in CD4+T cells isolated from the genital tract of stressed and non-stressed mice. Amplified Opti-4CN Substrate Kit from Bio-Rad was used to detect protein concentration following the manufacturer’s instructions. Relative protein expression was calculated as described in materials and methods. Data shown are a representative of two or more independent experiments performed on different dates.

Fig 3: Fold-changes of gene expression of transcription factors and signature cytokines of Th1 or Th2.A) GATA-3, B) T-bet, C) IL-4) and D) IFN-γ in naïve CD4+ T cells isolated from spleen cells normalized to the internal control gene, GAPDH compared to the non-stressed mice are shown. Data shown a mean +/_ SEM of two or more independent experiments ran on different dates. *Denotes significant statistical differences between treatment groups at the level of (p < 0.05).

Fig 4: The persistence of the effect of CIS on the modulation of transcription factors and signature cytokines of Th1 and Th2 cells in the genital tract of stressed/non-stressed mice.Gene expression of A), GATA-3, B) T-bet, IL-4 C), and D) IFN-γ during two or 11-days post-C. muridarum genital infection relative to non-infected mice. Results are mean +/- SEM of two independently performed experiments. *Denotes significant statistical differences between treatment groups at the level of (p < 0.05).