Fig 2: CASC9 functions as ceRNA TK1 in BC cells. (a) The TK1 expression was detected by qPCR in 5637cells transfected with CASC9 siRNAs or cotransfected with CASC9 siRNAs and miR-195-5p inhibitors. (b) The TK1 expression was detected by qPCR in 5637cells transfected with CASC9 vectors or cotransfected with CASC9 vectors and miR-195-5p mimics. (c) Dual luciferase reporter assays were used to determine the miR-195-5p binding on the 3'UTR of TK1. (d) The TK1 expression was detected by Western blot in 5637cells with indicated treatment. (e) QRT-PCR analysis of the TK1 expression in 30 pairs of BC and corresponding adjacent normal tissues. (f) Correlation analysis between TK1 and miR-195-5p in 30 paired BC samples (r = 0.-501, P < 0.001). (g) Correlation analysis between TK1 and CASC9 in 30 paired BC samples (r = 0.480, P < 0.001). **P < 0.01 and ***P < 0.001.