Fig 2: Ectoine enhances production of transmembrane mucins in DS mice. (A, C, E, G) Representative images of IF staining showing the decreased production of MUC1 (A), MUC4 (C), MUC16 (E), and MUC15 (G) in conjunctiva and/or cornea of DS mice (DS+PBS) was recovered by ectoine eye drops (DS+Ect); Scale bars = 50 µm. The stitched images were made because the cornea and conjunctiva in some eyeball sections were too far apart to be captured on the same image. (B, D, F, H) IF intensity quantified the alternation of these four mucins in the three groups; each circle or triangle represents one eye. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.