Fig 2: SOX10 was a direct target for miR‐637 in glioma cells. (A–D) The expression levels of SOX10 were measured by qRT‐PCR (A,B) or by Western blotting (C,D) in LN229 or SHG‐44 cells treated with miR‐637 mimics or inhibitor. (E) Bioinformatic analysis identified a potential binding site between miR‐637 and the 3’‐UTR sequences of SOX10. (F) The luciferase activity was measured by reporter assay. Wild‐type (WT) or mutated (MUT) SOX10 binding sequence was cloned into the reporter construct, co‐transfected into LN229 or SHG‐44 cells with either miR‐637 mimics or inhibitor. Data are presented as mean ± SD from three independent experiments. Comparison between two groups was performed using Student’s t‐test and between three or more groups using one‐way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01.

Fig 3: Immunohistochemistry of the mass in the lumbar spinal cord. (A–F) Representative images (A, C, E) and signal intensities (B, D, F) of oligodendrocyte transcription factor 2 (OLIG2), SRY-box transcription factor 10 (SOX10) and glial fibrillary acidic protein (GFAP) staining. (G) Representative images of Ki-67. (H and I) Ki-67 expression profiles in all tumor cells (H) and in each tumor cell type (I). The graphs were created based on the average percentages ± SD. Large=large cells, Small=small cells. Bars=20 µm.

Fig 4: CircEPHB4 and SOX10 were up‐regulated, while miR‐637 was down‐regulated in glioma tissues or cell lines. (A) The expression levels of circEPHB4, miR‐637 and SOX10 were examined by qRT‐PCR in 40 pairs of tumor‐free normal tissues and glioma tissues. (B) The relative expression of circEPHB4, miR‐637 and SOX10 examined in (A) is presented for each individual patient. (C) The correlations between the expression levels of circEPHB4 and miR‐637, circEPHB4 and SOX10, and miR‐637 and SOX10 were analyzed by Spearmen correlation analysis. (D) The correlations between the level of circEPHB4, miR‐637, and SOX10 and the overall survival of glioma patients were analyzed using Kaplan–Meier method. (E) TGCA dataset analyzed by Jefferson online tool (http://genomics.jefferson.edu/proggene/index.php) revealed significant correlation between high SOX10 expression and the reduced overall survival (3 and 5 years) of glioma patients (n = 103). (F,G) SOX10 expression was measured on the protein level by Western blotting and compared between NHA cells and three glioma cell lines A172, SHG‐44 and LN229 cells. (H) The expression levels of circEPHB4, miR‐637 and SOX10 were examined by qRT‐PCR and compared between NHA cells and three glioma cell lines A172, SHG‐44, and LN229. Data are presented as mean ± SD from three independent experiments. Comparison between two groups was performed using Student’s t‐test and between three or more groups using one‐way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01 and ***P < 0.001.

Fig 5: Effect of MLT+Ptero on apoptosis-related proteins in colon cancer cells. HCT116, SW480, and HT29 colon cancer cells were exposed to MLT (0.5, 1 mM) and/or Ptero (20, 40 µM) for 24 h and then were subjected to Western blotting for PARP, cleaved caspse-3, Bcl-xL, NEDD9, SOX10, SOX9, and β-actin.