Fig 2: IL-6 and IL-8 activate the NF-κB pathway. With the addition of IL-6 (200 ng/ml) and IL-8 (10 ng/ml) to LoVo-NC and HT29-P, NF-κB pathway activation was detected. p-IKKa relative to IKKa. **P<0.01. PRL-3, phosphatase of regenerating liver-3; HT29-P, HT29 PRL-3 knockdown; NC, negative control; IL, interleukin; p, phosphor; IKKα, nuclear factor κ-B kinase subunit α; VEGF, vascular endothelial growth factor.

Fig 3: The levels of colonic TLR4 and related protein expression in Tlr4fl/fl and Tlr4ΔIEC offspring mice with or without prenatal LPS exposure. (A) The levels of colonic TLR4 protein expression in offspring mice (n = 4 per group). (B) The levels of colonic CHUK, RELA, P-RELA, and PTGS2 protein expression in the four groups (n = 4 per group). (C) The TLR4, CHUK, and PTGS2 protein expression levels in the four groups were normalized to ACTB, and the P-RELA to RELA ratio was used to evaluate the proportion of P-RELA quantitatively (n = 4 per group; two-way ANOVA). Data were presented as mean ± standard error of the mean. The term “Interaction” denotes the effect of LPS in the Tlr4ΔIEC mice compared with Tlr4fl/fl mice. ns, not significant; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. TLR4, toll-like receptor 4; LPS, lipopolysaccharide; CHUK, component of inhibitor of nuclear factor Kappa B kinase complex; PTGS2, prostaglandin-endoperoxide synthase 2; ACTB, actin beta.