Fig 2: Effects of C. sinensis extract pretreatment (25 and 50 μg/mL) for 4 h on the regulation of (A) mRNA expression of type 1 collagen (COL1A1) and elastin (ELN) and (B) mRNA expression of MMP9 and MMP1; protein levels of COL1A1 (C), ELN (D), MMP1 (E), and MPP9 (F) after exposure to UVB (25 and 50 mJ/cm2) in HFF1 cells (G). Representative immunoblotting image of each protein examined. The data are reported as the mean ± S.D. from two experiments performed in quadruplicates. Dashes to the right of the membrane bands show positions of prestained molecular mass markers. ** Significant vs. untreated control cells: p < 0.01; *** Significant vs. untreated control cells: p < 0.001; # Significant vs. untreated irradiated cells: p ≤ 0.05; ## Significant vs. untreated irradiated cells: p < 0.01.

Fig 3: 3D docking conformation of SAC with (A) KDR, (B) IGF1 (C) PPARG (D) MMP1, respectively. The green sticks represent SAC.

Fig 4: Analysis of metastasis-associated genes in TAGLN2-knockdown cancer cells. (A) RT-qPCR analysis of ICAM1, VCAM1, IL6, CXCL1, CCL2, CXCR4, CCR4, MMP1, MMP2, and MMP9 mRNA expression in MDA-MB-231 cells treated with scramble or shTAGLN2; results are presented relative to β-actin expression. Data shown was representative of three independent experiments. (B) RT-qPCR analysis of ICAM1, VCAM1, IL6, CXCL1, CCL2, CXCR4, CCR4, MMP1, MMP2 and MMP9 mRNA expression in MCF-7 cells treated with shControl or shTAGLN2; results are presented as relative to β-actin expression. Data are representative of three independent experiments. (C) Immunoblotting of CXCR4, MMP1 and MMP2 expression in MDA-MB-231 and MCF-7 cells treated with scramble or shTAGLN2. Western blots were representative of three independent experiments. (D) RT-qPCR analysis of MMP1 and MMP2 in MDA-MB-231 cells transfected with scramble or shTAGLN2 under treatment of DMSO or SN50 (18 µM) for 24 h. Data are representative of three independent experiments. *P<0.05 vs. scramble or as indicated. CCL2, C-C motif chemokine ligand 2; CXCL1, C-X-C motif chemokine ligand 1; CCR4, C-C chemokine receptor type 4; CXCR4, including C-X-C chemokine receptor 4; DMSO, dimethyl sulfoxide; ICAM1, intercellular adhesion molecule 1; IL6, interleukin-6; MMP, matrix metalloproteinase; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; shRNA, short hairpin RNA; TAGLN2, transgelin 2; VCAM1, vascular cell adhesion protein 1.

Fig 5: Representative images from western blot for the proteins (A) and relative expression levels of VEGFR2 (B), MMP1 (C), PPARγ (D), and IGF1 (E). **p < 0.01, vs the sham group. # p < 0.05, ## p < 0.01, vs the I/R group (n = 3).