Description
Each antibody in the AMPK Substrate Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-AMPKα (Thr172) (D4D6D) Rabbit mAb and
AMPKα (D5A2) Rabbit mAb detects both α1 and α2 isoforms of the catalytic subunit. Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb detects endogenous levels of ULK1 only when phosphorylated at Ser555 of mouse ULK1 (equivalent to Ser556 of human ULK1). Bands of unknown origin are detected between 90 and 100 kDa. Phospho-Raptor (Ser792) Antibody detects non-specific signals of various molecular weights. Phospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb recognizes single phosphorylation of Ser93 to a greater extent than dual phosphorylation at Ser93 and Ser96. This antibody may also weakly detect an unidentified band at approximately 70 kDa