Fig 1: (A): Intron retention due to PRMT5 inhibition was evaluated by re-analysis of RNA-seq data (as described earlier) using IRFinder (n = 2 for each condition and per cell line). Volcano plot shows IRFinder output in JJN3 cells, identifying different gene targets affected by intron retention. Only the plot for JJN3 is shown here, other plots are shown in the supplementary data. (B): Venn diagram presentation of common genes affected by intron retention (n = 2 × 2 per HMCL/treatment condition). A set of 45 common affected genes were identified. Samples were analysed per cell line type to account for cell type specific effects. (C): Reactome enrichment analysis was subsequently performed using the 45 common genes. Selected pathways that were deregulated are shown. (D): Western blot analysis of identified targets MMSET/WHSC1 HELLS and SLAMF7 in JJN3, OPM2, XG7 and AMO1 cells. For MMSET, * depicts the wild type MMSET isoform where § depicts the aberrant MMSET protein affected by t (4; 14), which is present in OPM2 cells. Tubulin and actin were used as loading control. One experiment representative of 3 experiments performed is shown (n = 3).