Integrin beta8 (D1V7M) Rabbit mAb 88300S from Cell Signaling Technology

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Cells
September 24 2023

Fig 1: FRA1 upregulation alters the adhesion profile of CGL1 cells. (A) RNA-seq mRNA expression analysis of integrin family gene members. CGL1FRA1 gene expression is represented as fold change ± SEM relative to CGL1dCas9 cells (n = 3; *, p < 0.05). FRA1 upregulation alters the expression of the ECM profile in CGL1 cells. (B) Representative Western blot images of integrin α4, integrin α11, and integrin β11 protein expression in CGL1dCas9 and CGL1FRA1 cells. α-Tubulin was used in internal loading control. (C) Bar graphs illustrate CGL1FRA1 protein expression relative to CGL1dCas9 cells (*, p < 0.05). (D) RNA-seq mRNA expression analysis of select extracellular matrix (ECM) network genes. CGL1FRA1 gene expression is represented as fold change ± SEM relative to CGL1dCas9 cells (n = 3; *, p < 0.05). (E) CGL1FRA1 and CGL1dCas9 cells were assessed for adhesion to assay plates coated with 7.5 ng/μL fibronectin or 5 ng/μL collagen. The dashed line indicates the adhesion of control CGL1dCas9 cells to fibronectin and collagen. CGL1FRA1 cell adhesion is represented as fold change ± SEM relative to CGL1dCas9 cells. FRA1 overexpression decreased CGL1 cell adhesion to fibronectin and collagen (n = 3; *, p < 0.05). Abbreviations: COL14A1 (collagen type XIV alpha 1 chain), COL16A1 (collagen type XVI alpha 1 chain), COL3A1 (collagen type III alpha 1 chain), COL4A5 (collagen type IV alpha 5 chain), COL8A1 (collagen type VIII alpha 1 chain), FN1 (fibronectin 1), ITGA1 (integrin subunit alpha 1), ITGA11 (integrin subunit alpha 11), ITGA2 (integrin subunit alpha 2), ITGA3 (integrin subunit alpha 3), ITGA4 (integrin subunit alpha 4), ITGA5 (integrin subunit alpha 5), ITGA6 (integrin subunit alpha 6), ITGAE (integrin subunit alpha E), ITGB1 (integrin subunit beta 1), ITGB4 (integrin subunit beta 4), ITGB5 (integrin subunit beta 5), ITGB6 (integrin subunit beta 6), ITGB8 (integrin subunit beta 8), LAMB2 (laminin subunit beta 2), MMP2 (matrix metallopeptidase 2), MMP3 (matrix metallopeptidase 3), TGFB1 (transforming growth-factor beta 1), and TGFBI (transforming growth-factor beta induced).
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Cells
September 24 2023

Fig 2: RNA-seq transcriptome analysis of CGL1FRA1 cells relative to CGL1dCas9 cells. (A) A volcano plot illustrating the 298 differentially expressed genes (DEG) between CGL1FRA1 and CGL1dCas9 cells (DEG criteria: fold change <−1.5 and >1.5 and FDR adjusted p-value < 0.05). The red and blue dots represent 124 upregulated genes and 174 downregulated genes, respectively, in CGL1FRA1 cells relative to CGL1dCas9 cells. The left and right sides of the plot depict highly dysregulated genes, while genes higher on the graph indicate increased statistical significance. (B) Validation of RNA-seq dataset using RT-qPCR analysis. Twelve representative genes were randomly selected from the RNA-seq analysis. This list included six DEGs (ITGA4, FOSL1, CD24, ITGβ8, FOS, and FN1) and six non-DEGs (CCND1, EGFR, JUN, ITGB1, LAMA1, and CTNNB1). The graph represents CGL1FRA1 gene expression relative to CGL1dCas9 (CGL1FRA1—#1 and CGL1FRA1—#3 represent unique gRNA variants). The mRNA fold change between RNA-seq and RT-qPCR analyses were similar in magnitude and significance across the twelve representative genes (n = 3; *, p < 0.05). (C) Top 15 upregulated genes and (D) top 15 downregulated genes identified from the RNA-seq analysis of CGL1FRA1 cells relative to CGL1dCas9 cells, presented with gene description, p-value (p), and ranked based on fold change (F.C.). Abbreviations: CCND1 (cyclin D1), CTNNB1 (catenin beta 1), EGFR (epidermal growth-factor receptor), FN1 (fibronectin 1), FOS (Fos proto-oncogene), FOSL1 (FOS like 1), ITGA4 (integrin subunit alpha 4), ITGB1 (integrin subunit beta 1), ITGB8 (integrin subunit beta 8), JUN (Jun proto-oncogene), and LAMA1 (laminin subunit alpha 1).

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Integrin beta8 (D1V7M) Rabbit mAb

The Integrin beta8 (D1V7M) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to Integrin beta 8, and ITGB8. This antibody recognizes Mouse antigen. The Integrin beta8 (D1V7M) Rabbit mAb Antibody has been shown to work in the following applications: Western Blot.

Description

Integrin β8 (D1V7M) Rabbit mAb recognizes endogenous levels of total integrin β8 protein

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Integrin beta8 (D1V7M) Rabbit mAb from Cell Signaling Technology

Description

Cell Signaling Technology

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Integrin beta8 (D1V7M) Rabbit mAb from Cell Signaling Technology

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