Fig 2: Schematic illustration of TRAF3IP3 serving as immune rheostat by inhibiting RLR dependent IFN-I pathway.TRAF3IP3 inhibits the RLR sensing pathway by targeting at node of TBK1 and TRAF3.

Fig 3: MST4 inhibits the binding of TRAF3 to MAVS. A Immunoblot analysis of lysates of 293 T cells transfected with Flag-TRAF3, Myc-MAVS, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. B Immunoblot analysis of lysates of 293 T cells transfected with Flag-IKKe, Myc-MAVS, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. C Immunoblot analysis of lysates of 293 T-sh-Control and 293 T-sh-MST4 cells infected with SeV for 8 h, followed by immunoprecipitation with control rabbit-IgG or anti-TRAF3 antibodies. Lysates and immunoprecipitation extracts were probed with MAVS and TRAF3 antibodies. D Immunoblot analysis of lysates of 293 T cells transfected with Flag-TRAF3, HA-MAVS 360–540, and increasing amounts of Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. E Immunoblot analysis of lysates of 293 T cells transfected with Flag-MAVS, Myc-TRAF3, or Myc-MST4 for 24 h, followed by immunoprecipitation with anti-Flag beads. Data information: The data are representative of at least three independent experiments