Fig 1: Cytokine array and Western blot analysis of inflammation-related markers in Panc-1 cell line. Significantly decreased level of IL-17a measured by cytokine array and NFkB by Western blot were detected after 48 h of 20× methyl-donor treatment (A–D). Decreased protein level of CD30 did not reach significance after 48 h methyl-donor treatment, either by cytokine array (A,B) or by Western blot, similar to STAT3 protein level (C,D). Each bar represents the average normalized density from at least 3 repeats ± SD. Statistical significance are plotted as *: p < 0.05; **: p < 0.01. 1× and 20×: concentration of methyl donors.