Fig 1: The proliferation and differentiation potentials of OPC clones derived from dorsal and ventral germinal zones. A) The average size of clones as indicated. Of note, OPC clones from the ventral origin eventually vanished from the brain. Error bars: mean ± SEM. Two‐way ANOVA followed by Tukey post‐hoc test, *p < 0.05, ****p < 0.0001. B) The proportion of OPC in each clone. Error bars: mean ± SEM. Kruskal–Wallis test followed by Dunn post‐hoc test. *p < 0.05, ****p < 0.0001. ns, not significant. C) Representative images of an oligodendrocyte derived from ventral OPC expressing CC1, the differentiated oligodendrocyte marker. Scale bars, 10 µm. D) Representative images of an oligodendrocyte derived from ventral OPC expressing MBP, the myelinating oligodendrocyte marker and the staining of Caspr, which is the marker protein of paranodes. Scale bars, 10 µm. E–I) The OPC percentage in each clone from different brain structures. N/A, not applicable, which means no clone was found in these areas of those groups. For dorsal groups, there were N = 15 clones for 24 h, N = 12 for 48 h, N = 16 for 7dpi, N = 12 for 12 dpi, N = 81 for 18 dpi, N = 60 for 60 dpi, and N = 61 for 180 dpi. For ventral groups, there were N = 9 clones for 24 h, N = 27 for 48 h, N = 17 for 7 dpi, N = 5 for 12 dpi, N = 72 for 18 dpi, and N = 89 for 60 dpi.