Fig 2: Effects of Anesthesia/Surgery/ICU on synaptophysin, microtubule-associated protein 2 and postsynaptic density protein 95 levels. (A–C) Protein levels of Syp (A: P = 0.002), MAP2 (B: P = 0.013), and PSD-95 (C: P = 0.003) were significantly decreased in A/S/I mice relative to controls. Two-tailed unpaired t-test (N = 5 A/S/I and N = 5 control mice). Syp, synaptophysin; MAP2, microtubule-associated protein 2; PSD-95, postsynaptic density protein 95. (D) Representative 20× magnification images of the distribution of PSD-95 immunostaining in sagittal CA1 and CA3 hippocampal sections from two control and two A/S/I mice. Arrows highlight differences in PSD-95 immunofluorescent staining. (E) Plot of STX1a, Ntrk1, Snca α, Syp, MAP2, and PSD-95 protein levels against individual mouse Z scores. Z scores from behavioral assessments obtained at the 24 h time point were used, since tissue collection for protein studies was performed immediately after this behavioral assessment. The vertical dotted line represents a protein level of 85% (expressed as change from control). ∗P < 0.05 and ∗∗P < 0.01, respectively.