Fig 1: Detection of tissue factor (TF) in mouse tissues using the Cell Signaling Technology antibody. (A) Proteins in lysates or (B) control or deglycosylated lysates from the indicated wild-type (WT) or low TF (LTF) mouse organs (40 μg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to membranes. TF was detected using the Cell Signaling Technology 44861 rabbit anti-mouse TF monoclonal antibody. The binding of the Cell Signaling Technology anti-TF antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody (Table 1). α-Actinin was used as a loading control and detected with an anti–α-actinin antibody (Table 1). The binding of the anti–α-actinin antibody was detected with a goat anti-rabbit immunoglobulin G horseradish peroxidase-linked antibody (Table 1). For imaging, the TF blots were exposed for (A) 2, (B, top panel) 1, or (B, middle panel) 15 seconds. For α-actinin, the blots were exposed for (A) 15 or (B) 30 seconds. All blots were visualized using the iBright FL1000 imaging system. ab, antibody; dgTF, deglycosylated tissue factor; gTF, glycosylated tissue factor; kDa, kilodalton.