mCherry (E5D8F) Rabbit mAb 43590S from Cell Signaling Technology

Product Specs
ItemmCherry (E5D8F) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number43590S
ReactivityAll
Research AreaRelated Products
ClonalityMonoclonal
ApplicationsWB, ICC, IF
HostRabbit
TargetmCherry bacteria
Quantity100 µl

Questions and Answers

Can this antibody be used for detecting mCherry-tagged proteins in various species?

Yes, the mCherry (E5D8F) Rabbit mAb has been shown to detect mCherry-tagged proteins in a wide range of species, including human, mouse, zebrafish, and various other mammals and non-mammalian species, as indicated by its broad reactivity profile.

What applications has this antibody been successfully utilized in?

The mCherry (E5D8F) Rabbit mAb has been successfully utilized in applications such as Western blotting, immunocytochemistry, and immunofluorescence, as evidenced by its use in multiple scientific publications.

Are there any specific studies that have utilized this antibody in their experiments?

Yes, the antibody has been cited in at least 20 scientific articles, including studies such as those by Deb Roy et al. () and Hegazy et al. (), where it was used for immunoprecipitation and Western blotting to analyze protein interactions and localization.

What is the recommended dilution for using this antibody in Western blotting?

In Western blotting applications, the mCherry (E5D8F) Rabbit mAb is typically used at a dilution of 1:1000, as noted in various experimental protocols within the literature.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The cytoskeleton contributes to abnormal genome–lamina interactions in LMNA -deficient cardiomyocytesJournal of Cell BiologyMay 4, 2026Kaitlyn M. Shen, Emily J. Shields, Vasia Barka, Ashley Karnay, Leonie M. Kurzlechner, Jennifer D. Yang, Qiaohong Wang, Benjamin W. Lee, Carmen Suay-Corredera, Son C. Nguyen, et al.(1:500 sc-376248; SantaCruz), Rabbit anti-TNNT2 (1:1000 ab45932; abcam), Rabbit anti-mCherry (1:1000 43590; Cell Signaling), Mouse anti-nesprin-1 (1:100 MABT843; EMD), Rabbit anti-H3K9me2 (1:1000 39239; ActiveMotif;

(2) Mucin-binding protein shuttles enable delivery of brain-targeted therapeuticsbioRxivApril 12, 2026Sophia M. Shi, Gabrielle S. Tender, Jian Xiong, Josephine K. Buff, Hannah I. Park, Justin H. Mendiola, Edward N. Wilson, Monther Abu-Remaileh, Carolyn R. Bertozzi, Tony Wyss-Coraymouse anti-CLTC (1:100, Thermo, MA1-065), rabbit anti-mCherry (1:100, Cell Signaling Technologies, 43590), rat anti-mCherry (1:100, Thermo, M11217), goat anti-RAB5 (1:100, LSBio, LS-B12415-300), mouse anti-EEA1

(3) HIV-1 Vpr is targeted for degradation by autophagyPLoS PathogMarch 2, 2026Yuexuan Chen, Susanne Klute, Anju Bansal, Konstantin Maria Johannes Sparrer, Ruth Serra-MorenoLC3B,1:1000,"Cell Signaling, #2775S" mCherry,Rabbit monoclonal (E5D8F) to mCherry,1:1000,"Cell Signaling, #43590" Mouse IgG,Goat anti-mouse (H + L) secondary HRP,1:2000,"ThermoFisher Sci, #31430" Mouse IgG 1,Goat

(4) Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particlesPLOS PathogensFebruary 23, 2026Makoto Kuroda, Peter J. Halfmann21-5.2.5, in-house), CD41 (1:1000, ab134131, Abcam), Beta actin (1:1000, ab8224, Abcam), mCherry (1:1000, 43590, Cell Signaling Technology), and GFP (1:1000, 2956, Cell Signaling Technology). Quantitative Reverse

(5) Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STINGCell ReportsJanuary 27, 2026Chen Cheng, Hsiang-Ting Lu, Shan Li, Zhongsheng You,Cell Signaling Technology,Cat# 12480; RRID: AB_2797933 Rabbit anti-mCherry,Cell Signaling Technology,Cat# 43590; RRID: AB_2799246 Rabbit LC3B Antibody,Novusbio,Cat# NB100-2220; RRID: AB_10003146 Mouse mAb anti

(6) K48-ubiquitin-dependent proteases cut-up post-ER proteinsNature CommunicationsJanuary 13, 2026Annabel Y. Minard, Stanley Winistorfer, Liping Yu, Robert C. Pipernonspecific binding; the effective dilution was ~1:1000. The rabbit monoclonal α-mCherry E5D8f (CST; Cat. 43590S) was used at 1:1000–1:2000. Vps10 was detected using a rabbit polyclonal antibody raised against aa

(7) DEAD-box ATPase–marked condensates coordinate compartmentalized translation and antibiotic persistenceSci AdvJanuary 2, 2026Ziyin Zhang, Daqian Li, Bo Zheng, Jia-feng Liuanalyzed with ImageJ software. Primary antibodies used in this study were mCherry (Cell Signaling, 43590), His-tag antibody (Sangon, D191001), anti-DnaK antibody (Abcam, ab80161), and anti-puromycin (AbClonal,

(8) Synchronization of the segmentation clock using synthetic cell–cell signalingGenes & DevelopmentJanuary 1, 2026Akihiro Isomura, Daisuke Asanuma, Ryoichiro Kageyamaprimary antibody (anti-mCherry monoclonal [clone E5D8F] rabbit antibody [Cell Signaling Technology 43590; RRID: AB_2799246] at 1:200 dilution) overnight at 4°C. After three washes with PBS, cells were incubated

(9) 4R-tau isoform induction via TDP-43 in neurons in response to insulin: converging signaling pathways with implications for neurodegenerative diseaseActa neuropathologica communicationsDecember 24, 2025Weissmann C, Castellanos LCS, Montes MM, Uruk G, Youssef H, Reichard RR, Gatto RG, Josephs KA.(Cell Signaling, #3449, 1:1000); pSer (Stressmarq, SPC-149, 1:1000 ); Rabbit anti-mCherry (Cell Signaling, #43590; 1:1000); mouse anti-GFP (Cell Signaling, #2955; 1:1000). Images were taken using the Fuji AI600 imaging

(10) Endocannabinoids Block Headache and Anxiety Comorbidity via Two-pronged Anterior Insular ProjectionsResearch (Wash D C)December 9, 2025Jiang Bian, Yue-Hui Zhang, Li Yin, Jian-Feng Li, Xia Zhang, Xu-Feng Xuab279289, Abcam), rabbit EGFP [1:100, 2555, Cell Signaling Technology (CST)] primary antibodies (1:100, 43590, CST), rabbit anti-mCherry primary antibodies (1:100, 43590, CST), donkey anti-rabbit Alexa Fluor 488

(11) HIF2α inhibits glutaminase clustering in mitochondria to sustain growth of clear cell renal cell carcinomaJCI InsightDecember 8, 2025Wencao Zhao, Sara M. Demczyszyn, Nathan J. Coffey, Yanqing Jiang, Boyoung Kim, Schuyler Bowers, Caitlyn Bowman, Michael C. Noji, Cholsoon Jang, M. Celeste Simon, et al.12957-1-AP), HIF1α (Novus Bio, NB100-296), HIF2α (CST, 7096s), MYC-tag (CST, 2278s), mCherry (CST, 43590), 14-3-3 (CST, 8312), and GAPDH (CST, 5174). Secondary antibodies that are conjugated with HRP were

(12) Epstein-Barr virus exploits desmocollin 2 as the principal epithelial cell entry receptorNature microbiologyNovember 1, 2025Wang H, Mou Z, Yeo YY, Ge Q, Liu X, Narita Y, Li Z, Wang C, Li W, Zhao KR, et al.PE-conjugated antibody (356804, Biolegend 1:50), GAPDH (sc-47724 HRP, Santa Cruz, 1:5,000), mCherry (43590, CST, 1:1,000), EBV-gH/gL (769C2, 1:3,000)35, EBV-gH/gL (769C5)35, EBV-gp350(72A1)44, EBV-gp350 (OT6–2,

(13) The ribonuclease DISL-2 mediates piRNA degradation in C. elegansCell ReportsOctober 28, 2025Benjamin Pastore, Hannah L. Hertz, Wen Tanganti-alpha-tubulin,Bio-Rad,Cat# MCA77G; RRID:AB_325003 Rabbit mAb anti-mCherry,Cell Signaling Technology,Cat# 43590; RRID:AB_2799246 Goat anti-Rabbit IgG H&L (HRP),Abcam,Cat# ab6721; RRID:AB_955447 Goat anti-Rat IgG H&L (HRP

(14) Activation of NF‐κB Signaling by Optogenetic Clustering of IKKα and βAdvanced BiologySeptember 1, 2025Alexandra Anna Maria Fischer, Markus Michael Kramer, Miguel Baños, Merlin Moritz Grimm, Manfred Fliegauf, Bodo Grimbacher, Gerald Radziwill, Sven Rahmann, Wilfried Webercatalog no. 9246) were diluted 1:1000 in blocking buffer. Anti‐mCherry (Cell signaling, catalog no. 43590) was used at a dilution of 1:2000, and anti‐GAPDH (Cell signaling, catalog no. 5174) and anti‐GFP (Cell signaling

(15) Liver CYP4A autophagic lysosomal degradation: A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction siteMolecular PharmacologySeptember 1, 2025Liang He, Doyoung Kwon, Michael J. Trnka, Yi Liu, Jade Yang, Kathy H. Li, Rheem A. Totah, Eric F. Johnson, Alma L. Burlingame, Maria Almira CorreiaNo. 12741, CST), Beclin-1 (CST, #3738), Atg5 (D5F5U; Rabbit mAb, CST, #12994), mCherry (E5D8F; CST, #43590), DYKDDDDK-Tag antibody (binds to the same epitope as Sigma’s Anti-FLAG M2 Antibody; CST, #2368), and

(16) Compromised PGF2α signaling in the paraventricular hypothalamic nucleus contributes to the central sensitization of the nitroglycerin-induced chronic migraine in male miceThe journal of headache and painAugust 6, 2025Hu X, Wu L, Wang L, Peng C, Tian Y, Zhu Q, Zhu H, Nie L, Yin L, Zhang Y, et al.used for immunofluorescence staining were as follows: rabbit anti-mCherry primary antibodies (1:100, 43590, Cell Signaling Technology), rabbit EGFP primary antibodies (1:100, 2555, Cell Signaling Technology),

(17) Short-term stress preconditioning activates sympathetic innervation in the testes to mitigate testicular ischemia-reperfusion injuryJ NeuroinflammationJuly 23, 2025Liyong Liu, Ruiming Xu, Heshe Li, Yuanchen Ma, Yue Shu, Hongjie Liang, Tao Wang, Jiang Hao, Weiqiang Li, Andy Peng Xiang, et al.1:1500), mouse anti-c-Fos antibody (Abcam, ab208942, 1:1000), and rabbit anti-mCherry antibody (CST, 43590, 1:100). Ganglion sections were incubated with primary antibody: rabbit anti-GFP (1:1000, cat. AB152,

(18) Light controls gene functions through alternative splicing in fungiProceedings of the National Academy of SciencesJuly 1, 2025Yifan Li, Huanhong Lu, Degang Guo, Xiaoyan Li, Fei Qi, Jian Zhang, Reinhard Fischer, Qirong Shen, Zhenzhong Yuthe standard procedure with anti-GFP (Yeasen, 31002ES60), anti-mCherry (Cell Signaling Technology, 43590S), or anti-β-Actin antibodies (Abmart, T40104S) at a dilution of 1:1000. Horseradish peroxidase (HRP)-conjugated

(19) Cholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafishNat CommunJune 6, 2025Sema Elif Eski, Jiarui Mi, Macarena Pozo-Morales, Gabriel Garnik Hovhannisyan, Camille Perazzolo, Rita Manco, Imane Ez-Zammoury, Dev Barbhaya, Anne Lefort, Frédérick Libert, et al.primary antibodies: mouse anti-Anxa4/2F11 (1:100, Abcam, ab71286), rabbit anti-mCherry (1:1000, CST, 43590) and for 3 h at 37 °C for mouse anti-pS6 (1:200, CST, 2215S). Secondary antibodies (Alexa Fluor 647

(20) Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch PlatformACS Synth BiolMay 16, 2025Catherine A. Hamann, Andrew Kjar, Hyosung Kim, Alan J. Simmons, Hannah J. Brien, Cheryl I. Quartey, Bonnie L. Walton, Ken S. Lau, Ethan S. Lippmann, Jonathan M. Brunger0.1% Triton-X100 and 5% FBS). The mCherry primary antibody (rabbit anti-mCherry [1:200; Cell Signaling 43590]) was applied for 1 h at room temperature before washing with PBS. The secondary antibody (AlexaFluor

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mCherry (E5D8F) Rabbit mAb from Cell Signaling Technology

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mCherry (E5D8F) Rabbit mAb from Cell Signaling Technology

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