Fig 1: H3.3K27M overexpression had no effect on the proliferation of glioma cells but enhanced their invasion and migration in vivo. Bioluminescent imaging (A) and quantification analysis (B) of nude mice showed that H3.3K27M had no distinct effect on the growth rate of tumors compared with the vector group and Myc-H3F3A group. (C) Kaplan–Meier survival curve of nude mice injected orthotopically. H3.3K27M reduced the survival ability of nude mice. (D) Representative images of H&E staining in brain tissue. The Myc-H3.3K27M group showed greater infiltration than the other two groups. The red circle indicates the infiltration site. The table shows the number of infiltrating cells and their distance from the primary tumor. In this experiment, one mouse was randomly selected from each group on the 18th, 21st and 24th days after glioma cell inoculation for sectioning and H&E staining. In the vector and wild-type H3 groups, we did not observe tumor cell infiltration, but the infiltration of tumor cells were identified in the sections from three mice in the H3.3K27M mutant group. * p < 0.05; ns indicates no statistical significance.