Anti-CD40 [5D12] MBS488353 from MyBioSource.com

Description

Recombinant monoclonal antibody to CD40. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5D12.
Anti-CD40 mAb 5D12, was generated by immunizing mice with sf9 insect cells expressing recombinant human CD40 and selected for the ability to bind EBV-immortalized human B cells. The variable regions from mu5D12 were cloned and used to construct chimeric humanized IgG4 5D12

Product Specs
ItemAnti-CD40 [5D12]
CompanyMyBioSource.com
Price $425.00Supplier Page View Company Product Page
Catalog NumberMBS488353
Quantity0.2 mg
ClonalityMonoclonal
ReactivityCynomolgus, Marmoset Monkey, Human
FormatPBS with 0.02% Proclin 300
ApplicationsBlock (BL), ELISA (EIA), Flow Cytometry (FC/FACS), Surface Plasmon Resonance (SPR), Immunoprecipitation (IP), Immunohistochemistry (IHC)
SpeciesHuman
Molecular Weight22,259 Da
TargetCD40
PurityProtein A Affinity Purified
NCBI Full Gene NameCD40 molecule
NCBI Gene AliasesTNFRSF5, CDW40, p50, Bp50
Conjugate/TagUnconjugated
IsotypeIgG4 kappa
Concentration1 mg/ml
Clone5D12

Specifications/Features

ch5D12 binds specifically to recombinant and native human CD40 ectodomain and has been shown to also bind CD40 in cynomolgus and marmoset monkeys. The antibody requires residues within D1 and D1/B2 for binding (Hager et al, 2003) (D1 domain is crucial for CD40L binding). Binding characteristics are very similar between humanized ch5D12 and mu5D12. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to CD40L, and this interaction is involved in B-cell activation and proliferation, antigen-presenting cell (APC) activation, initiation of antigen-specific T-cell responses, immunoglobulin production, activation of effector macrophages isotype switching, homotypic adhesion and rescue from apoptosis

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