Mouse IgG2a Isotype Control (Clone: 231) MBS668899 from MyBioSource.com

Description

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens

Product Specs
ItemMouse IgG2a Isotype Control (Clone: 231)
CompanyMyBioSource.com
Price $175.00Supplier Page View Company Product Page
Catalog NumberMBS668899
Quantity0.1 mg
ApplicationsImmunohistochemistry (IHC), Flow Cytometry (FC/FACS)
SpeciesMouse
TargetIgG2a
PurityPurified
Affinity Chromatography
Conjugate/TagUnconjugated
Clone231
IsotypeIgG2a
Format25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic
ClonalityMonoclonal

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Questions and Answers

How can I effectively use this isotype control in my flow cytometry experiments?

The Mouse IgG2a Isotype Control is designed to help estimate non-specific binding of primary antibodies to cell surface antigens in flow cytometry. It is crucial to include this control to differentiate between specific and non-specific staining, ensuring that any observed signal is due to the specific antibody rather than background noise.

Is this isotype control suitable for immunohistochemistry applications?

Yes, the Mouse IgG2a Isotype Control has been shown to be applicable for use in immunohistochemistry. It serves as a valuable tool to assess the specificity of your primary antibodies by providing a baseline for non-specific binding in tissue samples.

What should I consider regarding the concentration of this isotype control in my assays?

The Mouse IgG2a Isotype Control is provided in a concentration of 0.1 mg in 50 µl or 0.2 mg in 200 µl. It is important to optimize the dilution based on your specific experimental conditions and the concentration of your primary antibodies to ensure accurate interpretation of results.

Can this isotype control be used with other species or is it specific to mouse samples?

This isotype control is derived from mouse immunoglobulin and is primarily intended for use with mouse primary antibodies. Its specificity means that it may not be suitable for experiments involving antibodies from other species, as the binding characteristics may differ significantly.