Fig 1: Surface GluA2 AMPA receptor in neonatal hippocampal neurons is decreased in A350V neurons and is increased after heat treatment. (A) Representative images (20× magnification) of total (T.GluA2) and surface (S.GluA2) GluA2 AMPA receptor in wild-type (WT) and mutant (A350V) IQSEC2 hippocampal neurons. Neurons treated with heat (40 °C) for 1 h are labelled as MUT.H.S or WT.H.S. (B) Quantification of relative surface GluA2 expression (ratio: S.GluA2/T.GluA2). Data are reported as the mean ± SEM obtained from at least 4 neuronal culture preparations for each of the 4 groups. ** p < 0.01, *** p < 0.001, n.s. p > 0.05.