Western blot All lanes: CALCOCO2 antibody at 3.36ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 48, 55, 56, 44 kDa Observed band size: 56 kDa
CALCOCO2 Antibody
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA614394ESR1HU at dilution of 1:100
Immunoprecipitating CALCOCO2 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA614394ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA614394ESR1HU (4μg) + Hela whole cell lysate (500μg) Lane 3: Hela whole cell lysate (20μg)
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