Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA022517LA01HU at dilution of 1:100

IHC image of CSB-PA022517LA01HU diluted at 1:150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western blot
All lanes: SPCS3 antibody at 0.1µg/ml + Mouse kidney tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa