Immunohistochemistry analysis of RMI2 in paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with RMI2 polyclonal antibody (Product # PA5-95632) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Western blot analysis of RMI2 in Lane 1: human HeLa whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with RMI2 polyclonal antibody (Product # PA5-95632) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).