Western blot was performed using Anti-ATF2 Recombinant Rabbit Monoclonal Antibody (Product # MA5-33115) and a 60 and 40kDa band corresponding to Cyclic AMP-dependent transcription factor ATF-2 was observed across all the tested cell lines and tissues, except Mouse Skeletal Muscle and Rat Skeletal Muscle. Nuclear enriched extracts (30 µg lysate) of FaDu (Fig. A, Lane 1), HeLa (Fig. A, Lane 2), HCT 116 (Fig. A, Lane 3), SK-O-V3 (Fig. A, Lane 4), Mouse Brain (Fig. A, Lane 5), Rat Brain (Fig. A, Lane 6), Mouse Skeletal Muscle (Fig. A, Lane 7), Rat Skeletal Muscle (Fig. A, Lane 8), and Whole cell extracts of iPSC (Fig. B, Lane 1), iPSC (differentiated to cardiomyocytes) (Fig. B, Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).