PLK3 Antibody PA5-97143 from Thermo Fisher Scientific | Biocompare.com

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PLK3 Antibody

Western blot analysis of PLK3 in extracts of various cell lines. Samples were incubated with PLK3 Polyclonal antibody (Product # PA5-97143) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
WB

Western Blot analysis of PLK3 in extracts of various cell lines using PLK3 Polyclonal Antibody (Product # PA5-97143) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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PLK3 Antibody

Thermo Fisher Scientific's PLK3 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, and Western Blot. The PLK3 Antibody was generated using PLK3 as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

Product Specs
ItemPLK3 Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-97143
Quantity100 µL
ApplicationsELISA, Western Blot
Concentration0.5 mg/mL
Conjugate/TagUnconjugated
FormatPBS with 50% glycerol and 0.02% sodium azide; pH 7.3
PurityAffinity Chromatography
ReactivityHuman, Mouse, Rat
TargetPLK3
IsotypeIgG
HostRabbit
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 487-646 of human PLK3 (NP_0040642)
Antibody TypePrimary
NCBI Full Gene Namepolo like kinase 3, polo-like kinase 3
NCBI Gene AliasesPLK-3, FNK, PRK, Cnk, Fnk, CNK
ClonalityPolyclonal
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

Thermo Fisher Scientific

Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) Parkin-mediated mitophagy is negatively regulated by FOXO3A, which inhibits Plk3-mediated mitochondrial ROS generation in STZ diabetic stress-treated pancreatic β cellsPloS oneJanuary 1, 2023Shim JY, Chung JO, Jung D, Kang PS, Park SY, Kendi AT, Lowe VJ, Lee S.

(2) Natural COA water inhibits mitochondrial ROS-mediated apoptosis through Plk3 downregulation under STZ diabetic stress in pancreatic β-cell linesBiochemistry and biophysics reportsJuly 1, 2022Lee J, Chung JO, Park SY, Rajamohan N, Singh A, Kim J, Lowe VJ, Lee S.monoclonal antibody recognizing Plk1 (35–206) and Rabbit polyclonal antibody recognizing Plk3 invitrogen (PA5-97143) were purchased from Invitrogen. Anti-beta-actin mouse antibodies were purchased from Sigma. Please

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