Immunohistochemistry analysis of ESD in paraffin-embedded human trachea. Samples were incubated with ESD Polyclonal antibody (Product # PA5-96537) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of ESD in paraffin-embedded Human trachea using ESD Polyclonal Antibody (Product # PA5-96537) at a dilution of 1:100.
Western blot analysis of ESD in extracts of various cell lines. Samples were incubated with ESD Polyclonal antibody (Product # PA5-96537) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supplier Page from Thermo Fisher Scientific for ESD Antibody