Western blot analysis of DACH1 in 293F cells. Samples were incubated with DACH1 Polyclonal antibody (Product # PA5-93268) using a dilution of 1:900, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.