Western blot analysis of FASTK in extracts of various cell lines. Samples were incubated with FASTK Polyclonal antibody (Product # PA5-96567) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry-Immunofluorescence analysis of FASTK was performed in U2OS cells using FASTK Polyclonal Antibody (Product # PA5-96567). Blue: DAPI for nuclear staining.
Supplier Page from Thermo Fisher Scientific for FASTK Antibody