Immunofluorescence analysis of DYRK1A in U20S cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with DYRK1A polyclonal antibody (Product # PA5-95499) at a 2 µg/mL dilution, followed by Cy3 conjugated Goat anti-rabbit IgG using a 1:100 dilution and DAPI.

Western blot was performed using Anti-DYRK1A Polyclonal Antibody (Product # PA5-95499) and a 90kDa band corresponding to Dual specificity tyrosine-phosphorylation-regulated kinase 1A was observed across all the tested cell lines and tissues, except HEK-293 and Mouse Brain. Nuclear enriched extracts (40 µg lysate) of HEK-293 (Lane 1), K-562 (Lane 2), HEL 92.1.7 (Lane 3), SH-SY5Y (Lane 4), PC-3 (Lane 5), HeLa (Lane 6), Jurkat (Lane 7), Mouse Ovary (Lane 8), Mouse Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).