Immunocytochemistry-Immunofluorescence analysis of ZWINT was performed in NIH-3T3 cells using ZWINT Polyclonal Antibody (Product # PA5-92903) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of ZWINT in extracts of various cell lines. Samples were incubated with ZWINT Polyclonal antibody (Product # PA5-92903) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.