Western blot analysis of RNF3 in extracts of various cell lines. Samples were incubated with RNF3 Polyclonal antibody (Product # PA5-96686) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Western Blot analysis of RNF3 in extracts of various cell lines using RNF3 Polyclonal Antibody (Product # PA5-96686) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supplier Page from Thermo Fisher Scientific for RNF3 Antibody