Immunohistochemistry-Immunofluorescence analysis of NF-H was performed in mouse brain tissue using NF-H Polyclonal Antibody (Product # PA5-97057) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of NF-H in extracts of various cell lines. Samples were incubated with NF-H Polyclonal antibody (Product # PA5-97057) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.