Immunohistochemical analysis of HDAC6 in paraffin embedded human liver cancer using a HDAC6 monoclonal antibody (Product # MA5-33150) at a dilution of 1:112.5. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.