Immunohistochemistry analysis of RPL4 in paraffin-embedded mouse brain. Samples were incubated with RPL4 Polyclonal antibody (Product # PA5-96464) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot analysis of RPL4 in extracts of various cell lines using RPL4 Polyclonal Antibody (Product # PA5-96464) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of RPL4 in extracts of various cell lines. Samples were incubated with RPL4 Polyclonal antibody (Product # PA5-96464) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Supplier Page from Thermo Fisher Scientific for RPL4 Antibody