C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Syrian Hamster IgG Isotype Control, PE-Cyanine5 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).