Human IL-28 R alpha /IFN-lambda R1 Antibody AF5260 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IL-28 R alpha /IFN-lambda R1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF5260
NCBI Gene AliasesCRF2/12, IL28RA, IFNLR, LICR2, IL-28R1
Quantity100 ug (also 25 ug)
ApplicationsFlow Cytometry,Western Blot,Neutralization,CyTOF-ready
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Primate - Chlorocebus aethiops (African Green Monkey)
TargetIL-28 R alpha/IFN-lambda R1
HostSheep
IsotypeIgG
NCBI Full Gene Nameinterferon lambda receptor 1
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1, Arg21-Ala228, Accession # Q8IU57
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for flow cytometry applications?

Yes, the IL-28 R alpha/IFN-lambda R1 antibody has been shown to work in flow cytometry applications. In a study, it was utilized to sort cells based on their expression levels of IFN-λR1, demonstrating its effectiveness in this technique ().

What species does this antibody react with?

The IL-28 R alpha/IFN-lambda R1 antibody is reactive with human, mouse, and non-human primate species, making it suitable for a variety of experimental models.

How does this antibody perform in Western blotting?

The IL-28 R alpha/IFN-lambda R1 antibody has been successfully used in Western blotting to detect human IL-28 R alpha, indicating its reliability for protein analysis in this application.

Is there any specific concentration or protocol recommended for using this antibody in cell sorting?

In the referenced study, a concentration of 5 μg of the IL-28 R alpha/IFN-lambda R1 antibody per 1 × 10^6 cells was used for cell sorting, which may serve as a guideline for similar applications.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Casein Kinase 1α Mediates the Degradation of Receptors for Type I and Type II Interferons Caused by Hemagglutinin of Influenza A VirusJournal of virologyApril 1, 2018Xia C, Wolf JJ, Vijayan M, Studstill CJ, Ma W, Hahm B.

(2) The IFN-λ-IFN-λR1-IL-10Rβ Complex Reveals Structural Features Underlying Type III IFN Functional PlasticityImmunityMarch 21, 2017Juan L. Mendoza et al.washed twice with cold PBS + 0.5% BSA. Cells were then incubated with anti-IL-28RA (R&D Systems; cat# AF5260) at 5 μg antibody per 1 × 106 cells in 0.4 mL volume for 30 min, washed 3 times, incubated with FITC

(3) A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infectionProceedings of the National Academy of Sciences of the United States of AmericaJanuary 24, 2017Saxena K, Simon LM, Zeng XL, Blutt SE, Crawford SE, Sastri NP, Karandikar UC, Ajami NJ, Zachos NC, Kovbasnjuk O, et al.

(4) Replication of Human Norovirus RNA in Mammalian Cells Reveals Lack of Interferon ResponseJournal of virologyOctober 1, 2016Qu L, Murakami K, Broughman JR, Lay MK, Guix S, Tenge VR, Atmar RL, Estes MK.anti-IL-10 receptor ␤ (IL10R2) (AF874; R&D Systems) at 1:100, sheep anti-IL-28 receptor ␣ (IL28R1) (AF5260; R&D Systems,) at 1:100, and a cocktail of the above three antibodies at the same dilutions for neutralization

(5) Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancerCancer researchJune 1, 2010Wongthida P, Diaz RM, Galivo F, Kottke T, Thompson J, Pulido J, Pavelko K, Pease L, Melcher A, Vile R.

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IL-28 R alpha/IFN-lambda R1 Antibody

IL-28 R alpha/IFN-lambda R1 Antibody

IL-28 R alpha/IFN-lambda R1 Antibody

IL-28 R alpha/IFN-lambda R1 Antibody

Human IL-28 R alpha /IFN-lambda R1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (5)

Reviews

Human IL-28 R alpha /IFN-lambda R1 Antibody from R&D Systems, a Bio-Techne Brand

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