ROBO3 Antibody AF3155 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemROBO3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF3155
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityMouse (Ms)
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived mouse ROBO3, Gly54-Ser545, Accession # Q9Z2I4
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameroundabout guidance receptor 3
NCBI Gene AliasesHGPPS, HGPPS1, HGPS, RBIG1, RIG1
Antibody TypePrimary Antibodies
PurityAntigen Affinity-purified
ConcentrationLYOPH
ApplicationsWB, IHC, IHC-fr, IHC-p, BL
HostGoat
IsotypeIgG
TargetROBO3

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse ROBO3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) ROBO3 and less than 5% cross-reactivity with rhROBO2 and rhROBO4 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for Western blotting to detect mouse ROBO3?

Yes, the ROBO3 antibody has been shown to work effectively in Western blotting applications for detecting mouse ROBO3.

What is the cross-reactivity of this antibody with human ROBO3?

The ROBO3 antibody exhibits approximately 40% cross-reactivity with recombinant human ROBO3, while showing less than 5% cross-reactivity with human ROBO2 and ROBO4.

Is this antibody suitable for immunohistochemistry applications?

Yes, the ROBO3 antibody is suitable for immunohistochemistry applications, including both frozen and paraffin-embedded tissue sections.

What is the source of the immunogen for this antibody?

The immunogen for the ROBO3 antibody is derived from a mouse myeloma cell line NS0, specifically targeting the mouse ROBO3 protein from Gly54 to Ser545.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Establishing the role of ZBTB20 mutations in GnRH deficiency and impaired neurogenesis in the subventricular zone: a human cohort and animal model studyEBioMedicineAugust 4, 2026Jun Guan, Jingtan Zhu, Yuting Li, Min Nie, Jing Zhang, Zhiheng Chen, Huadie Liu, Dan-Na Chen, Ruizhi Zheng, Meichao Men, et al.Rabbit anti-ZBTB20 (1:500, Cat# 23987-1-AP, Proteintech, RRID:AB_2879389); Goat anti-Robo3 (1:300, AF3155, R&D systems, RRID:AB_10644167); Rat anti-PHH3 (1:400, ab10543, Abcam, RRID:AB_2295065); Rabbit anti-Ki67

(2) Thyroid hormone rewires cortical circuits to coordinate body-wide metabolism and exploratory drivebioRxiv : the preprint server for biologyAugust 10, 2023Hochbaum DR, Dubinsky AC, Farnsworth HC, Hulshof L, Kleinberg G, Urke A, Wang W, Hakim R, Robertson K, Park C, et al.

(3) Cbln1 regulates axon growth and guidance in multiple neural regionsPLoS biologyNovember 1, 2022Han P, She Y, Yang Z, Zhuang M, Wang Q, Luo X, Yin C, Zhu J, Jaffrey SR, Ji SJ.

(4) NELL2-Robo3 complex structure reveals mechanisms of receptor activation for axon guidanceNature communicationsMarch 20, 2020Pak JS, DeLoughery ZJ, Wang J, Acharya N, Park Y, Jaworski A, Özkan E.

(5) A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout ReceptorsThe Journal of biological chemistryMay 29, 2015Samelson BK, Gore BB, Whiting JL, Nygren PJ, Purkey AM, Colledge M, Langeberg LK, Dell'Acqua ML, Zweifel LS, Scott JD.

(6) Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axonsDevelopment (Cambridge, England)March 1, 2014Inamata Y, Shirasaki R.

(7) Slit2 and Robo3 modulate the migration of GnRH-secreting neuronsDevelopment (Cambridge, England)September 1, 2012Cariboni A, Andrews WD, Memi F, Ypsilanti AR, Zelina P, Chedotal A, Parnavelas JG.

(8) Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expressionNeuronAugust 12, 2010Kuwako K, Kakumoto K, Imai T, Igarashi M, Hamakubo T, Sakakibara S, Tessier-Lavigne M, Okano HJ, Okano H.

(9) The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowtheLifeJanuary 1, 0001"Mondal B, Jin H, Kallappagoudar S, Sedkov Y, Martinez T, Sentmanat MF, Poet GJ, Li C, Fan Y, Pruett-Miller SM, et al."antibodies directed against the extracellular domain of the axon guidance receptors ROBO3 (R&D Systems, AF3155), UNC5B (R&D Systems, MAB1006), a combination of ROBO3/UNC5B or IgG (Millipore, 12–370) as a negative

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ROBO3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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ROBO3 Antibody from R&D Systems, a Bio-Techne Brand

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