Human LDLR Antibody AF2148 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman LDLR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2148
NCBI Gene AliasesFH, LDLCQ2, FHCL1, FHC
ApplicationsBlocking,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,Functional Assay
Conjugate/TagUnconjugated
IsotypeIgG
HostGoat
TargetLDLR
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHamster,Human,Mouse,Primate - Cercopithecus aethiops (African Green Monkey),Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Rat
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human LDL R, Asp193-Arg788, Accession # P01130
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namelow density lipoprotein receptor

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What species does this antibody react with, and has it been used in relevant studies?

The LDLR antibody has been shown to react with multiple species, including human, mouse, rat, non-human primates, and hamster. It has been utilized in various studies, such as those investigating LDLR expression in pancreatic cancer cells and its role in atherosclerosis, demonstrating its applicability in diverse biological contexts.

Can this antibody be used for immunoblotting, and what are some examples of its application in research?

Yes, the LDLR antibody has been successfully used in immunoblotting. For instance, it was employed in studies to analyze LDLR levels in various cell types, including in the context of PCSK9 regulation and its effects on LDLR degradation, as well as in the evaluation of LDLR expression in induced pluripotent stem cell-derived endothelial cells.

Is there any information on the specificity of this antibody in experimental settings?

The LDLR antibody has been shown to specifically detect human LDLR in direct ELISAs and Western blots, with approximately 15% cross-reactivity with recombinant mouse LDLR. This specificity is crucial for accurately assessing LDLR levels in various experimental models.

How has this antibody been validated in knockout or knockdown studies?

The LDLR antibody has been validated in knockout and knockdown studies, confirming its effectiveness in detecting LDLR protein levels in various experimental setups. This validation supports its reliability for use in studies focused on LDLR function and regulation in different biological systems.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) In Vivo Determinants of Hepatitis C Virus Adaptation and Escape From Neutralizing Antibody AR5ACellular and Molecular Gastroenterology and HepatologyJanuary 1, 2026Rodrigo Velázquez-Moctezuma, Rani Burm, Lieven Verhoye, Laura Collignon, Kenn Holmbeck, Erick Giang, Mansun Law, Jens Bukh, Philip Meuleman, Jannick Prentoeanti-CD81 (BD Pharminogen, Cat. JS81), anti-SR-BI, C16-17,51,68 and polyclonal anti-LDLr (R&D Systems, Cat. AF2148). Control antibodies for receptor blocking were antibody 55344 for CD81 (BD Pharminogen), the antibody

(2) Hijacking Extracellular Targeted Protein Degrader-Drug Conjugates for Enhanced Drug DeliveryJ Am Chem SocOctober 29, 2025Fangzhu Zhao, Yan Wu, Kaitlin Schaefer, Yun Zhang, Kun Miao, Zi Yao, Snehal D. Ganjave, Kaan Kumru, Trenton M. Peters-Clarke, Alex Inague, et al.α-tubulin (Cell Signaling Technology, 3873S, 1:3000), goat anti-human LDLR (R&D Systems, catalog no. AF2148, 1:1000), IRDye 800CW goat anti-rabbit IgG (LI-COR Biosciences, catalog no. 926-32211), IRDye 680RD

(3) Lipid raft proteomics identify endothelial myosin-9 (MYH9) as a regulator of low-density lipoprotein transcytosis and atherosclerosisProceedings of the National Academy of SciencesOctober 28, 2025Erika Jang, Siavash Ghaffari, Andria Henry, Grace Wen, Tse Wing Winnie Ho, Wan Hei Cheng, Yunfan Lyu, Kuiru Wei, Changsen Wang, Casey E. Romanoski, et al.with 1/1,000 primary antibody on a shaker overnight at 4 °C. The antibodies used were goat anti-LDLR (AF2148, R&D Systems), mouse anti-cav-1 (610407, BD Biosciences), rabbit anti-CD36 (NB400-144, Novus Bio), goat

(4) LDL transcytosis passes through the trans-Golgi network and requires Rab10Journal of lipid researchOctober 1, 2025Winnie Ho TW, Wang C, Lee WL.overnight at 4°C. Immunoblotting was performed with the following primary antibodies: goat anti-LDLR (AF2148; R&D Systems), rabbit anti-SR-BI (NB400-104; Novus Bio), rabbit anti-ALK1 (70R-49334; Fitzgerald), mouse

(5) Low-density lipoprotein receptor-targeting chimeras for membrane protein degradation and enhanced drug deliverybioRxivJune 13, 2025Fangzhu Zhao, Yan Wu, Kaitlin Schaefer, Yun Zhang, Kun Miao, Zi Yao, Snehal D. Ganjave, Kaan Kumru, Trenton M. Peters-Clarke, Alex Inague, et al.anti-human β-tubulin (Cell Signaling Technology, 3873S, 1:3000), goat anti-human LDLR (R&D Systems, Cat#AF2148, 1:1000), IRDye 800CW goat anti-rabbit IgG (LI-COR Biosciences, Cat# 926-32211), IRDye 680RD goat anti-mouse

(6) Decreased lipidated ApoE-receptor interactions confer protection against pathogenicity of ApoE and its lipid cargoes in lysosomesCellJanuary 9, 2025Jing L. Guo, Dylan Braun, Gabriel A. Fitzgerald, Yun-Ting Hsieh, Lionel Rougé, Alexandra Litvinchuk, Micah Steffek, Nicholas E. Propson, Catherine M. Heffner, Claire Discenza, et al.surface LDLR was performed by incubating live cells with an anti-human LDLR antibody (R&D Systems, AF2148) diluted 1:200 in live staining medium (cold Opti-MEM supplemented with 25 mM HEPES) for 1 h at 4°C.

(7) The low-density lipoprotein receptor and apolipoprotein E associated with CCHFV particles mediate CCHFV entry into cellsNature communicationsMay 28, 2024Ritter M, Canus L, Gautam A, Vallet T, Zhong L, Lalande A, Boson B, Gandhi A, Bodoirat S, Burlaud-Gaillard J, et al.

(8) Insights into PCSK9-LDLR Regulation and Trafficking via the Differential Functions of MHC-I Proteins HFE and HLA-CCellsMay 17, 2024Mikaeeli S, Ben Djoudi Ouadda A, Evagelidis A, Essalmani R, Ramos OHP, Fruchart-Gaillard C, Seidah NG.(NEB-cell signaling, Ontario, Canada #3267T [1:1000]), hPCSK9 (in house [1:2000]), LDLR (R&D system #AF2148 [1:1000])), V5 (Invitrogen, Waltham, MA, USA #R960-25 [1:5000]), monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse

(9) A high-cholesterol diet promotes the intravasation of breast tumor cells through an LDL-LDLR axisScientific reportsApril 24, 2024Magalhães A, Cesário V, Coutinho D, Matias I, Domingues G, Pinheiro C, Serafim T, Dias S.

(10) iPSC-Derived Endothelial Cells Reveal LDLR Dysfunction and Dysregulated Gene Expression Profiles in Familial HypercholesterolemiaInternational journal of molecular sciencesJanuary 5, 2024Zakharova IS, Shevchenko AI, Arssan MA, Sleptcov AA, Nazarenko MS, Zarubin AA, Zheltysheva NV, Shevchenko VA, Tmoyan NA, Saaya SB, et al.IgG,1/400 Immunoblotting,Immunoblotting,Immunoblotting,Immunoblotting,Immunoblotting,Immunoblotting LDLR,R&D System,AF2148,AB_2135126,goat IgG,1/1000 ACTB,Abcam,ab8227,AB_2305186,rabbit IgG,1/5000 goat IgG + peroxidase,JacksonImmunoResearch,705-035-003,AB_2340390,donkey

(11) Identification of PCPE-2 as the endogenous specific inhibitor of human BMP-1/tolloid-like proteinasesNature communicationsDecember 4, 2023Vadon-Le Goff S, Tessier A, Napoli M, Dieryckx C, Bauer J, Dussoyer M, Lagoutte P, Peyronnel C, Essayan L, Kleiser S, et al.was a kind gift of Dr. Larry W. Fisher (NIH, Bethesda, USA; dilution 1/1000). LDLR was detected with AF2148 (dilution 1/2000), human PCPE-1 with AF2627 (dilution 1/1000), mouse PCPE-1 with AF2239 (dilution 1/1000)

(12) The Apolipoprotein E neutralizing antibody inhibits SARS-CoV-2 infection by blocking cellular entry of lipoviral particlesMedComm (2020)October 1, 2023Cui Q, Jeyachandran AV, Garcia G, Qin C, Zhou Y, Zhang M, Wang C, Sun G, Liu W, Zhou T, et al.specific for Spike (1:100; Sino Biological; Catalog # 40150‐R007), LDLR (1:25; R&D System; Catalog # AF2148), and ACE2 (1:100; Sigma; Catalog # AMAB91262) were used for IHC staining. 4.8 Antibody The antibody

(13) A virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in non-human primatesNPJ vaccinesSeptember 28, 2023Fowler A, Van Rompay KKA, Sampson M, Leo J, Watanabe JK, Usachenko JL, Immareddy R, Lovato DM, Schiller JT, Remaley AT, et al.(catalogue number AF2255; R&D Systems) or goat anti-human LDLR at a 1:500 dilution (catalogue number AF2148; R&D Systems) followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary

(14) Proteolysis of the low‐density lipoprotein receptor in hepatocytes is mediated by BMP1 but not by other astacin proteasesFEBS LettersJune 5, 2023Katherine A. B. Kellett, Kate Fisher, Harry Aldworth, Nigel M. Hooperwere incubated overnight at 4 °C in primary antibody diluted in 2% BSA in PBS‐T: goat anti‐human LDLR (AF2148), goat anti‐mouse LDLR (AF2255; R&D Systems, Minneapolis, MN, USA), chicken anti‐LDLR (Ab14056; Abcam,

(15) A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human PrimatesbioRxiv : the preprint server for biologyMay 15, 2023Fowler A, Van Rompay KKA, Sampson M, Leo J, Watanabe JK, Usachenko JL, Immareddy R, Lovato DM, Schiller JT, Remaley AT, et al.

(16) Lipoproteins act as vehicles for lipid antigen delivery and activation of invariant natural killer T cellsJCI insightMay 8, 2023Engelen SE, Ververs FA, Markovska A, Lagerholm BC, Kraaijenhof JM, Yousif LI, Zurke YX, Gulersonmez CM, Kooijman S, Goddard M, et al.on a 96-well plate (Corning). Upon preincubation with 5 μg/mL LDLR-blocking antibody for 30 minutes (AF2148, R&D Systems, Bio-Techne), THP1 cells were incubated for 4 hours with 0.3 μL (6 μg/mL) or 0.03 μL (0.6

(17) Targeting UGCG Overcomes Resistance to Lysosomal Autophagy InhibitionCancer discoveryFebruary 6, 2023Jain V, Harper SL, Versace AM, Fingerman D, Brown GS, Bhardwaj M, Crissey MAS, Goldman AR, Ruthel G, Liu Q, et al.Flotillin 1 (610820, BD Transduction Laboratories), Flotillin-2 (sc-28320, Santa Cruz Biotechnology), LDLR (AF2148, R&D Systems), CerSyn2 (SAB2101321, Sigma-Aldrich), Cl-Cas-3 (9661, Cell Signaling Technology); SR-B1

(18) Altered cell function and increased replication of rhinoviruses and EV-D68 in airway epithelia of asthma patientsFrontiers in microbiologyJanuary 1, 2023Essaidi-Laziosi M, Royston L, Boda B, Pérez-Rodriguez FJ, Piuz I, Hulo N, Kaiser L, Clément S, Huang S, Constant S, et al.then incubated with Anti-ICAM1 (diluted 1/500, Abcam, ab2213), -LDL-R (diluted 1/1,000, R&D systems, AF2148), CDHR3 (diluted 1/500, Sigma-Aldrich, HPA011218), and Actin (diluted 1/1,000 Millipore, MAB1501) primary

(19) Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearanceEMBO reportsFebruary 3, 2022Chen Y, Wu X, Zhang J, Pan G, Wang X, Guo X, Wang J, Cui X, Gao H, Cheng M, et al.

(20) Downregulation of low-density lipoprotein receptor mRNA in lymphatic endothelial cells impairs lymphatic function through changes in intracellular lipidsTheranosticsJanuary 1, 2022Vachon L, Smaani A, Tessier N, Jean G, Demers A, Milasan A, Ardo N, Jarry S, Villeneuve L, Alikashani A, et al.

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LDLR Antibody

LDLR Antibody

LDLR Antibody

Human LDLR Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (50)

Reviews

Human LDLR Antibody from R&D Systems, a Bio-Techne Brand

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