Nectin-4 Antibody AF2659 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemNectin-4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2659
NCBI Gene AliasesLNIR, nectin-4, EDSS1, PRR4, PVRL4
ApplicationsWestern Blot,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry,CyTOF-ready,Functional Assay
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityCanine,Hamster,Human,Mouse,Porcine,Primate - Chlorocebus aethiops (African Green Monkey),Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Xenograft
HostGoat
IsotypeIgG
TargetNectin-4
NCBI Full Gene Namenectin cell adhesion molecule 4
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human Nectin-4, Gly27-Val351, Accession # Q96NY8
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Nectin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse Nectin-4 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry in canine tissues?

Yes, the Nectin-4 antibody has been shown to work in immunohistochemistry for canine prostate tissues, as demonstrated in a study evaluating Nectin-4 expression in various canine prostate conditions, including benign prostatic hyperplasia and prostatic carcinoma ().

Is there any evidence of Nectin-4 being involved in cancer progression?

Yes, Nectin-4 has been identified as a tumor-associated marker in various cancers. For instance, it has been associated with metastatic potential in canine prostate tumors and is considered a prognostic factor in early breast cancer, where its expression correlates with disease-free survival rates (, ).

How does the Nectin-4 antibody perform in flow cytometry applications?

The Nectin-4 antibody has been utilized in flow cytometry to assess Nectin-4 expression in engineered tumor cell lines, confirming its effectiveness in detecting Nectin-4 levels in various experimental setups ().

What types of cancer have been linked to Nectin-4 expression in research?

Nectin-4 expression has been linked to several cancers, including breast cancer, where it has been shown to promote anchorage-independent growth, and malignant mesothelioma, where it is not required for measles virus entry but is involved in the tumor microenvironment (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Immunohistochemical, Immunofluorescent, and Molecular Investigation of Canine Distemper Virus, Nectin 4, and CD150 Receptors in Naturally Infected Dogs with Canine Distemper VirusAnimals (Basel)July 18, 2026Hilmi Nuhoğlu, Enver BeytutMA1-82327; Thermo Fisher Scientific, Waltham, MA, USA), Human Nectin 4 Polyclonal Antibody (Cat. No. AF2659; R&D Systems, Minneapolis, MN, USA), Recombinant Anti-SLAM/CD150 Antibody (Cat. No. 10837-R008; Sino Biological Inc

(2) Exploring membranous NECTIN‐4 expression patterns and enfortumab vedotin response in prostate cancerJournal of Cellular and Molecular MedicineJuly 28, 2024Richard Weiten, Marit Bernhardt, Max Niemann, Glen Kristiansen, Viktor Grünwald, Manuel Ritter, Michael Hölzel, Markus Eckstein, Abdullah Alajati, Niklas Klümper, et al.Tween 20, pH 7.5) for 60 min and then incubated overnight at 4°C with primary antibodies against NECTIN‐4 (#AF2659, R&D Systems, Wiesbaden‐Nordenstadt, Germany, dilution 1:500) and β‐Actin (#A2228, Sigma dilution, Taufkirchen,

(3) Anti-tumor activity of a recombinant measles virus against canine lung cancer cellsScientific reportsOctober 24, 2023Tamura K, Fujiyuki T, Moritoh K, Akimoto H, Iizuka K, Sato H, Asano K, Yoneda M, Kai C.

(4) Immune response elicited in the tumor microenvironment upon rMV-SLAMblind cancer virotherapyCancer scienceMay 1, 2023Moritoh K, Shoji K, Amagai Y, Fujiyuki T, Sato H, Yoneda M, Kai C.on 10% polyacrylamide gels and transferred to PVDF membranes. Human anti‐NECTIN4 antibody (1:1000, AF2659; R&D systems) and HRP‐conjugated anti‐goat IgG antibody (1:2000; Dako) were used to detect NECTIN4;

(5) LRP6 Is a Functional Receptor for Attenuated Canine Distemper VirusmBioFebruary 28, 2023Gradauskaite V, Inglebert M, Doench J, Scherer M, Dettwiler M, Wyss M, Shrestha N, Rottenberg S, Plattet P.postseeding, the cells were treated with 4% PFA. To detect Nectin-4 (N4), cross-reacting anti-human N4 Abs (AF2659, R&D Systems, 1 h) (or goat serum [X0907, Dako] as a control) were used at concentrations of 5 μg/mL.

(6) Development of Nectin4/FAP-targeted CAR-T cells secreting IL-7, CCL19, and IL-12 for malignant solid tumorsFrontiers in immunologyJanuary 1, 2022Li F, Zhao S, Wei C, Hu Y, Xu T, Xin X, Zhu T, Shang L, Ke S, Zhou J, et al.stained with 10 μg/ml primary goat anti-human Nectin4, affinity-purified polyclonal antibody (Catalog # AF2659, R&D Systems) at 4°C overnight. Then, the sections were stained with the secondary biotinylated rabbit

(7) Anticancer immunity induced by a synthetic tumor-targeted CD137 agonistJournal for immunotherapy of cancerJanuary 1, 2021Upadhyaya P, Lahdenranta J, Hurov K, Battula S, Dods R, Haines E, Kleyman M, Kristensson J, Kublin J, Lani R, et al.selected with Blasticidin. Several individual clones were screened by flow cytometry (anti-Nectin-4, AF2659, R&D Systems) to select clones with appropriate level of Nectin-4 expression. Clones CT26#7 and MC38#13

(8) Antitumor activity of an oncolytic measles virus against canine urinary bladder transitional cell carcinoma cellsResearch in veterinary scienceDecember 1, 2020Iizuka K, Shoji K, Fujiyuki T, Moritoh K, Tamura K, Yoshida A, Sato H, Yoneda M, Asano K, Kai C.

(9) Nectin-4 and p63 immunohistochemical expression in canine prostate tumourigenesisVeterinary and comparative oncologySeptember 1, 2019Della Salda L, Massimini M, Romanucci M, Palmieri C, Perillo A, Grieco V, Malatesta D, Spinillo MA, Passantino G, Dondi F, et al.was performed using the following primary antibodies (Abs): goat polyclonal antihuman Nectin-4 (1:70, AF2659, R&D Systems, Minneapolis, MN) and mouse monoclonal anti-human p63 (1:400, clone 4A4, DAKO, Glostrup,

(10) Over-expression of Nectin-4 promotes progression of esophageal cancer and correlates with poor prognosis of the patientsCancer cell internationalJanuary 1, 2019Deng H, Shi H, Chen L, Zhou Y, Jiang J.tissue sections at 100 °C for 30 min in EDTA solution (pH 9.0). Goat anti-human Nectin-4 antibody (AF2659, R&D Systems) was used at the concentration of 10 μg/mL. The Polink-2 plus® polymer HRP detection system

(11) Localization of nectin-2α at the boundary between the adjacent somata of the clustered cholinergic neurons and its regulatory role in the subcellular localization of the voltage-gated A-type K(+) channel Kv4.2 in the medial habenulaThe Journal of comparative neurologyJune 15, 2018Shiotani H, Miyata M, Itoh Y, Wang S, Kaito A, Mizoguchi A, Yamasaki M, Watanabe M, Mandai K, Mochizuki H, et al.mouse brains (Honda et al., 2006). Goat 28 anti-nectin-4 pAb (R&D Systems, Minneapolis, MN; Cat# AF2659, RRID: 29 AB_416659) recognized a 66-kDa band in Western blotting of the of pregnant 30 mouse mammary

(12) Cap-dependent translational control of oncolytic measles virus infection in malignant mesotheliomaOncotargetSeptember 8, 2017Jacobson BA, Sadiq AA, Tang S, Jay-Dixon J, Patel MR, Drees J, Sorenson BS, Russell SJ, Kratzke RA.isotype IgG (R & D Systems, catalog number AB-108-C), or nectin-4 IgG (R & D Systems, catalog number AF2659) or CD46 IgG (Santa Cruz, Dallas TX, catalog number sc-5267-clone m177) or both CD46 and nectin-4 antibodies.

(13) Expression of canine distemper virus receptor nectin-4 in the central nervous system of dogsScientific reportsMarch 23, 2017Pratakpiriya W, Ping Teh AP, Radtanakatikanon A, Pirarat N, Thi Lan N, Takeda M, Techangamsuwan S, Yamaguchi R.

(14) A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland DevelopmentThe Journal of biological chemistryMarch 11, 2016Kitayama M, Mizutani K, Maruoka M, Mandai K, Sakakibara S, Ueda Y, Komori T, Shimono Y, Takai Y.

(15) The Nectin-4/Afadin Protein Complex and Intercellular Membrane Pores Contribute to Rapid Spread of Measles Virus in Primary Human Airway EpitheliaJournal of virologyJuly 1, 2015Singh BK, Hornick AL, Krishnamurthy S, Locke AC, Mendoza CA, Mateo M, Miller-Hunt CL, Cattaneo R, Sinn PL.2a01:4f9:3a:1a84::2. afadin (dilution, 1:200; R&D Systems, Minneapolis, MN), human nectin-4 (1:200; catalog no. AF2659; R&D Systems, Minneapolis, MN), ZO-1 (1:500; Thermo Fisher Scientific, Waltham, MA), or N protein (1:500; in-house-generated

(16) SLAM- and nectin-4-independent noncytolytic spread of canine distemper virus in astrocytesJournal of virologyMay 1, 2015Alves L, Khosravi M, Avila M, Ader-Ebert N, Bringolf F, Zurbriggen A, Vandevelde M, Plattet P.tissue sections were incubated overnight at 4°C with the first polyclonal antibody, anti-nectin-4 (AF2659; R&D Systems) at a concentration of 1:1,000 in PBS-T under a humid atmosphere. Successively, biotinylated

(17) Nectin-4 expression contributes to tumor proliferation, angiogenesis and patient prognosis in human pancreatic cancerJournal of experimental & clinical cancer research : CRMarch 28, 2015Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y.to the manual provided by the manufacturer. As primary antibodies, the anti-human Nectin-4 antibody (AF2659, 1:40 dilution; R&D Systems, USA) was used. The anti-human CD4, CD8, CD45RO, CD31, and Ki67 antibodies

(18) Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancerCancer researchJanuary 1, 2015Galanis E, Atherton PJ, Maurer MJ, Knutson KL, Dowdy SC, Cliby WA, Haluska P Jr, Long HJ, Oberg A, Aderca I, et al.Cat# H-5000). Immunochemistry for nectin-4 The primary antibody Nectin4 MAB2659 (R&D Systems; Cat# AF2659) was diluted 1:500, and the slides were incubated overnight, at 4°C in a humidified chamber. For the

(19) Membranous Nectin-4 expression is a risk factor for distant relapse of T1-T2, N0 luminal-A early breast cancerOncogenesisSeptember 1, 2014Lattanzio R, Ghasemi R, Brancati F, Sorda RL, Tinari N, Perracchio L, Iacobelli S, Mottolese M, Natali PG, Piantelli M.protein, respectively. We used a commercially available, affinity purified polyclonal goat antibody (AF2659; R&D Systems), produced by immunization with recombinant human Nectin-4 from Gly27 to Val351, also including

(20) Measles vaccine strains for virotherapy of non-small-cell lung carcinomaJournal of thoracic oncology : official publication of the International Association for the Study of Lung CancerAugust 1, 2014Patel MR, Jacobson BA, Belgum H, Raza A, Sadiq A, Drees J, Wang H, Jay-Dixon J, Etchison R, Federspiel MJ, et al.next day, cells were treated with Isotype IgG (R & D systems, AB-108-C), Nectin-4 IgG (R & D systems, AF2659) 14, CD46 IgG (Santa Cruz, sc-5267-clone m177), or both Nectin-4 and CD46 Abs at a concentration of

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