Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl 401215 from BioLegend

Product Specs
ItemUltra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl
CompanyBioLegend
Price $110.00Supplier Page View Company Product Page
Catalog Number401215
Quantity100 µg
ApplicationsFC - Quality tested, ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Conjugate/TagUnconjugated, Ultra Low Endotoxin Azide Free
Format0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
IsotypeMouse IgG2b, kappa
CloneMG2b-57
HostMouse
ImmunogenTrinitrophenol + KLH
ClonalityMonoclonal
Antibody TypeIsotype Control
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Spatiotemporal profiling reveals distinct dynamics and checkpoint regulations of CAR-T and CAR-NKT cells against solid tumorsSignal Transduct Target TherMarch 12, 2026Yan-Ruide Li, Miao Li, Yuning Chen, Houfu Leng, Yichen Zhu, Xinyuan Shen, Duncan Croll, Enbo Zhu, Jie Huang, Yu-Chen Wang, et al.BioLegend), or Ultra-LEAFTM purified mouse lgG2bk isotype control antibody (Clone MG2B-57, cat. no. 401217, RRID: AB_3097073, BioLegend) was added to co-cultures one hour prior to adding CAR-NKT cells. In vitro

(2) CD8+ T-cell Antitumor Immunity via Human iNKT–DC ConjugatesCancer Immunology ResearchFebruary 3, 2026Dana C. Baiu, Kelsey A. Smith, Sarah A. Ferguson, Nathan R. Schwartz, Shashwot Tripathy, Joshua Brand, Huy Q. Dinh, Makoto Ohashi, Eric C. Johannsen, Jenny E. GumperzIgG1 clone MOPC-21 (BioLegend Cat# 400165, RRID:AB_11150399) and IgG2b clone MG2b-57 (BioLegend Cat# 401215, RRID:AB_3097073). Murine xenograft model of human EBV-driven B cell lymphoma. Animal husbandry and

(3) Tumor-specific CD8 T cell characterization in HR+ breast cancer reveals an impaired antitumoral response in patients with lymph node metastasisCell Reports MedicineAugust 19, 2025Mariana Pereira Pinho, Elie Antoun, Balraj Sandhar, Ting Shu, Fei Gao, Xiaobao Yang, Adam Bates, Lucia Cerundolo, Megat H.B.A. Hamid, David Maldonado-Perez, et al.400264; RRID:AB_11148947 "Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody",Biolegend,Cat# 401215; RRID:AB_3097073 ,, Biological samples,Biological samples,Biological samples ,, Human Blood and

(4) Down-Regulation of HLA-C Expression on Melanocytes May Contribute to the Therapeutic Efficacy of UVB Phototherapy in PsoriasisInternational Journal of Molecular SciencesMarch 21, 2025Yukiyasu Arakawa, Akiko Arakawa, Seçil Vural, Mengwen He, Sigrid Vollmer, Jörg C. PrinzSigma Aldrich) were stained with antibodies for HLA-C, HLA-ABC, or corresponding isotype control (BioLegend #401216, #400264, #311441) and anti-mouse IgG (H + L) Alexa Fluor 488 or 594 secondary antibody (Invitrogen

(5) Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinomaFrontiers in immunologyJanuary 1, 2024Sakata T, Yoshio S, Yamazoe T, Mori T, Kakazu E, Aoki Y, Aoyanagi N, Okamoto T, Ito T, Toyoda H, et al.incubated with an ILT2-blocking antibody (10 μg/mL) (333721; Biolegend) or isotype antibody (10 μg/mL) (401216; Biolegend) for 1 h. Pre-treated NK cells (effector cells) were incubated with CFSE-labeled K562 and

(6) Structure of the lysosomal mTORC1-TFEB-Rag-Ragulator megacomplexNatureFebruary 1, 2023Cui Z, Napolitano G, de Araujo MEG, Esposito A, Monfregola J, Huber LA, Ballabio A, Hurley JH.(catalogue no. 901513) was from Biolegend and HRP-conjugated secondary antibodies to mouse (catalogue no. 401215, 1:5,000 dilution) and rabbit (catalogue no. 401315, 1:5,000 dilution) IgGs were from Calbiochem. Chemicals

(7) Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old miceScience advancesOctober 21, 2022Sanz-Ros J, Romero-García N, Mas-Bargues C, Monleón D, Gordevicius J, Brooke RT, Dromant M, Díaz A, Derevyanko A, Guío-Carrión A, et al.secondary antibody (anti-rabbit; Cell Signaling Technology, 70745; 1:2000 dilution; anti-mouse, BioLegend, 401215; 1:1000 dilution) for 60 min at RT. Following three washes with TBS-t, membranes were developed with

(8) ERAP1 Controls the Autoimmune Response against Melanocytes in Psoriasis by Generating the Melanocyte Autoantigen and Regulating Its Amount for HLA-C*06:02 PresentationJournal of immunology (Baltimore, Md. : 1950)November 1, 2021Arakawa A, Reeves E, Vollmer S, Arakawa Y, He M, Galinski A, Stöhr J, Dornmair K, James E, Prinz JC.cells were reacted with antibodies for HLA-C, HLA-ABC or corresponding isotype control (Biolegend #401216, #400264, #311441, and DT-9, all without azide), and anti-mouse IgG (H+L) Alexa Fluor 488 or 594 secondary

(9) Inherited PD-1 deficiency underlies tuberculosis and autoimmunity in a childNature medicineSeptember 1, 2021Ogishi M, Yang R, Aytekin C, Langlais D, Bourgey M, Khan T, Ali FA, Rahman M, Delmonte OM, Chrabieh M, et al.Cat: A01829, 82 μg), Ultra-LEAF purified mouse IgG2b isotype control (BioLegend, Clone: MG2b-57, Cat: 401215, 82 μg), and mouse IgG2b anti-PD-1 antibody (MyBioSource, Clone: 9X21, Cat: 690864, 82 μg). On the next

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Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl from BioLegend

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Ultra-LEAF™ Purified Mouse IgG2b, κ Isotype Ctrl from BioLegend

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